Chaperones of the endoplasmic reticulum are required for Ve1‐mediated resistance to Verticillium. (10th September 2013)

• Record Type: Journal Article
• Authors: Liebrand, Thomas W. H.; Kombrink, Anja; Zhang, Zhao; Sklenar, Jan; Jones, Alexandra M. E.; Robatzek, Silke; Thomma, Bart P. H. J.; Joosten, Matthieu H. A. J.
• Is Part Of: Molecular plant pathology. Volume 15:Number 1(2014:Jan.)
• Page Start: 109
• Page End: 117
• Publication Date: 2013-09-10
• Subjects: Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12071
• Languages: English
• ISSNs: 1464-6722

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>The tomato receptor‐like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen <italic>Verticillium dahliae</italic>. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1‐enhanced green fluorescent protein (eGFP) from <italic>Nicotiana benthamiana</italic> leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)‐resident chaperones HSP70 binding proteins (BiPs) and a lectin‐type calreticulin (CRT). Knock‐down of the different <italic>BiP</italic>s and <italic>CRT</italic>s in tomato resulted in compromised Ve1‐mediated resistance to <italic>V. dahliae</italic> in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP‐type <italic>Cladosporium fulvum</italic> resistance protein Cf‐4 of tomato, as silencing of <italic>CRT3a</italic> resulted in a reduced pool of complex glycosylated Cf‐4 protein. In contrast, knock‐down of the various <italic>CRT</italic>s in <italic>N. benthamiana</italic> or <italic>N. tabacum</italic> did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf‐4‐ and Ve1‐mediated immunity.</p> </abstract>