Further research on the biological function of inclusion bodies of Anomala cuprea entomopoxvirus, with special reference to the effect on the insecticidal activity of a Bacillus thuringiensis formulation. Issue 1 (26th April 2013)

• Record Type: Journal Article
• Authors: Mitsuhashi, Wataru; Asano, Shoji; Miyamoto, Kazuhisa; Wada, Sanae
• Is Part Of: Pest management science. Volume 70:Issue 1(2014:Jan.)
• Page Start: 46
• Page End: 54
• Publication Date: 2013-04-26
• Subjects: Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.3521
• Languages: English
• ISSNs: 1526-498X

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="ps3521-sec-0001" sec-type="section"> <title>Background</title> <p> <bold>Entomopoxviruses (EVs) form two types of inclusion body: spheroids, which contain virions, and spindles, which do not. The authors tested whether the spindles from a coleopteran EV, <italic>Anomala cuprea</italic> EV (ACEV), enhanced the insecticidal activity of a commercial <italic>Bacillus thuringiensis</italic> (<italic>Bt</italic>) formulation and the susceptibility of scarabaeid pest species in Japan to the virus's spheroids, to assess whether ACEV inclusion bodies are potential biological control agents for pest insects</bold>.</p> </sec> <sec id="ps3521-sec-0002" sec-type="section"> <title>Results</title> <p> <bold>Peroral inoculation with both ACEV spindles and the <italic>Bt</italic> toxin only or the complete <italic>Bt</italic> formulation shortened the survival and increased the mortality of treated insects compared with those of insects inoculated with <italic>Bt</italic> without the spindles (8–38 h of decrease in LT<sub>50</sub> values among assays). ACEV showed high infectivity to a major scarabaeid pest species in Japanese sugar cane fields</bold>.</p> </sec> <sec id="ps3521-sec-0003" sec-type="section"> <title>Conclusion</title> <p> <bold>The results suggest that spindles or the constituent protein fusolin can be used as a coagent with <italic>Bt</italic> formulations, and that fusolin coexpression with a <italic>Bt</italic> toxin in crops might improve the insecticidal efficacy. In addition, the spheroids are potential biocontrol agents for some scarabaeid pests that are not easy to control because of their underground habitation. © 2013 Society of Chemical Industry</bold> </p> </sec> </abstract>