Noninvasive fetal RHD genotyping by microfluidics digital PCR using maternal plasma from two alloimmunized women with the variant RHD(IVS3+1G>A) allele. (19th September 2013)

• Record Type: Journal Article
• Authors: Tsui, Nancy B. Y.; Hyland, Catherine A.; Gardener, Glenn J.; Danon, David; Fisk, Nicholas M.; Millard, Glenda; Flower, Robert L.; Dennis Lo, Y. M.
• Is Part Of: Prenatal diagnosis. Volume 33:Number 12(2013:Dec.)
• Page Start: 1214
• Page End: 1216
• Publication Date: 2013-09-19
• Subjects: Prenatal diagnosis -- Periodicals; Fetus -- Diseases -- Diagnosis -- Periodicals; Electronic journals; 618.32075
• DOI: 10.1002/pd.4230
• Languages: English
• ISSNs: 0197-3851

Abstract

<abstract abstract-type="short"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>What's already known about this topic?</bold> <list id="pd4230-list-0001" list-type="bullet"> <list-item id="pd4230-li-0001"> <p>Noninvasive prenatal <italic>RHD</italic> typing can be achieved by using cell‐free fetal DNA in the plasma of RhD‐negative mothers.</p> </list-item> <list-item id="pd4230-li-0002"> <p>For RhD‐negative mothers carrying intact but dysfunctional <italic>RHD</italic> gene variants, the abundant maternal <italic>RHD</italic> sequences in maternal plasma could interfere with the fetal <italic>RHD</italic> allele detection.</p> </list-item> </list> </p> <p> <bold>What does this study add?</bold> <list id="pd4230-list-0002" list-type="bullet"> <list-item id="pd4230-li-0003"> <p>Digital PCR provides a high analytical specificity to noninvasively determine the fetal inheritance of <italic>RHD</italic> allele in alloimmunized pregnancies involving maternal <italic>RHD</italic> variants.</p> </list-item> </list> </p> </abstract>