Principal component analysis of odor coding at the level of third‐order olfactory neurons in Drosophila. (7th October 2013)

• Record Type: Journal Article
• Authors: Hiroi, Makoto; Ohkura, Masamichi; Nakai, Junichi; Masuda, Naoki; Hashimoto, Koichi; Inoue, Kiichi; Fiala, André; Tabata, Tetsuya
• Is Part Of: Genes to cells. Volume 18:Number 12(2013:Dec.)
• Page Start: 1070
• Page End: 1081
• Publication Date: 2013-10-07
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12094
• Languages: English
• ISSNs: 1356-9597

Abstract

<abstract abstract-type="main" id="gtc12094-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Olfactory information in <italic>Drosophila</italic> is conveyed by projection neurons from olfactory sensory neurons to Kenyon cells (KCs) in the mushroom body (MB). A subset of KCs responds to a given odor molecule, and the combination of these KCs represents a part of the neuronal olfactory code. KCs are also thought to function as coincidence detectors for memory formation, associating odor information with a coincident punishment or reward stimulus. Associative conditioning has been shown to modify KC output. This plasticity occurs in the vertical lobes of MBs containing α/α' branches of KCs, which is shown by measuring the average Ca²⁺ levels in the branch of each lobe. We devised a method to quantitatively describe the population activity patterns recorded from axons of &gt;1000 KCs at the α/α' branches using two‐photon Ca²⁺ imaging. Principal component analysis of the population activity patterns clearly differentiated the responses to distinct odors.</p> </abstract>