Partial sequence and characterization of nitric oxide synthase gene from Hyphantria cunea. Issue 6 (25th November 2013)

• Record Type: Journal Article
• Authors: Kim, Hong Ja; Kim, Yong Il; Kwon, Yong Min; Kang, Young Jin; Cheon, Hyang Mi; Ha, Nam Gyu; Seo, Sook Jae
• Is Part Of: Entomological research. Volume 43:Issue 6(2013:Nov.)
• Page Start: 344
• Page End: 352
• Publication Date: 2013-11-25
• Subjects: Insects -- Korea -- Periodicals; Entomology -- Periodicals; 595.709519
• DOI: 10.1111/1748-5967.12040
• Languages: English
• ISSNs: 1738-2297

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Nitric oxide synthase (NOS) gene has been partially sequenced from <italic>Hyphantria cunea</italic> and compared with those already determined from insects. <italic>Hyphantria cunea</italic> NOS possesses putative recognition sites for co‐factors heme, BH₄, CaM, FMN, FAD, and NADPH common to NOS. The deduced amino acid sequence of <italic>H. cunea</italic> NOS cDNA showed 70.3% identity to <italic>Manduca sexta</italic> NOS and 57.6–69.5% identity to NOS sequences from other insects. Nitric oxide synthase is expressed in all tissues of <italic>H. cunea, </italic> except in hemocytes. The NOS expression in midgut, fat body, epidermis, and Malpighian tubule strongly increased against Gram‐positive and Gram‐negative bacterial infection. These results suggest that NOS may play an important role in insect defense system against bacterial infection.</p> </abstract>