Molecular arrangement between multivalent glycocluster and Pseudomonas aeruginosa LecA (PA‐IL) by atomic force microscopy: influence of the glycocluster concentration1. Issue 12 (26th November 2013)

• Record Type: Journal Article
• Authors: Sicard, D.; Chevolot, Y.; Souteyrand, E.; Imberty, A.; Vidal, S.; Phaner‐Goutorbe, M.
• Is Part Of: Journal of molecular recognition. Volume 26:Issue 12(2013:Dec.)
• Page Start: 694
• Page End: 699
• Publication Date: 2013-11-26
• Subjects: Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2333
• Languages: English
• ISSNs: 0952-3499

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>New therapeutics strategy against cystic fibrosis seeks to prevent the adhesion of the bacterium <italic>Pseudomonas aeruginosa</italic> (PA) on the epithelial cells in the lungs. One of the factors that induces the adhesion is the interaction between natural glycocluster present on the cells and lectins such as the PA lectin LecA (PA‐IL) present on the bacterium. By introducing synthetic glycoclusters with a great affinity with the lectin PA‐IL, the adhesion can be prevented. In this study, we characterized, by atomic force microscopy, the interaction between a tetra‐galactosylated glycocluster and the PA‐IL lectin for high concentration of lectins (2.5 μM).We showed that the strong lectin/lectin interaction is reduced even for low concentration of glycoclusters (1 for 20 000 lectins). We assumed that it is due to the tensioactive behavior of the glycoclusters. It was shown that the arrangement of the created complexes induced different structures evolving from one‐dimensional elongated aggregates to two‐dimensional compact islands when increasing the glycocluster concentration. This evolution can be interpreted as the predominance of the glycocluster/lectin interaction. Copyright © 2013 John Wiley &amp; Sons, Ltd.</p> </abstract>