Combinatorial functions of diverse histone methylations in Arabidopsis thaliana flowering time regulation. Issue 1 (18th September 2013)

• Record Type: Journal Article
• Authors: Shafiq, Sarfraz; Berr, Alexandre; Shen, Wen‐Hui
• Is Part Of: New phytologist. Volume 201:Issue 1(2014)
• Page Start: 312
• Page End: 322
• Publication Date: 2013-09-18
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12493
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12493-abs-0001"> <title>Summary</title> <p> <list id="nph12493-list-0001" list-type="bullet"> <list-item> <p>Previous studies in <italic>Arabidopsis thaliana</italic> have identified several histone methylation enzymes, including ARABIDOPSIS TRITHORAX1 (ATX1)/SET DOMAIN GROUP 27 (SDG27), ATX2/SDG30, LSD1‐LIKE1 (LDL1), LDL2, SDG8, SDG25, and CURLY LEAF (CLF)/SDG1, as regulators of the key flowering repressor <italic>FLOWERING </italic><italic>LOCUS </italic><italic>C</italic> (<italic>FLC</italic>) and the florigen <italic>FLOWERING </italic><italic>LOCUS </italic><italic>T</italic> (<italic>FT</italic>). However, the combinatorial functions of these enzymes remain largely uninvestigated.</p> </list-item> <list-item> <p>Here, we investigated functional interplays of different histone methylation enzymes by studying higher order combinations of their corresponding gene mutants.</p> </list-item> <list-item> <p>We showed that H3K4me2/me3 and H3K36me3 depositions occur largely independently and that SDG8‐mediated H3K36me3 overrides ATX1/ATX2‐mediated H3K4me2/me3 or LDL1/LDL2‐mediated H3K4 demethylation in regulating <italic>FLC</italic> expression and flowering time. By contrast, a reciprocal inhibition was observed between deposition of the active mark H3K4me2/me3 and/or H3K36me3 and deposition of the repressive mark H3K27me3 at both <italic>FLC</italic> and <italic>FT</italic> chromatin; and the double mutants <italic>sdg8 clf</italic> and <italic>sdg25 clf</italic> displayed enhanced early‐flowering phenotypes of the respective single mutants.</p> </list-item> <list-item> <p>Collectively, our results provide important insights into the interactions of different types of histone methylation and enzymes in the regulation of <italic>FLC</italic> and <italic>FT</italic> expression in flowering time control.</p> </list-item> </list> </p> </abstract>