Comparison of neuroprotective effects of extract and fractions from Agarum clathratum against experimentally induced transient cerebral ischemic damage. (March 2014)

• Record Type: Journal Article
• Authors: Kim, In Hye; Yoo, Ki-Yeon; Park, Joon Ha; Yan, Bing Chun; Ahn, Ji Hyeon; Lee, Jae-Chul; Kwon, Hyuk Min; Kim, Jong-Dai; Kim, Young-Myeong; You, Sang Guan; Kang, Il-Jun; Won, Moo-Ho
• Is Part Of: Pharmaceutical biology. Volume 52:Number 3(2014:Mar.)
• Page Start: 335
• Page End: 343
• Publication Date: 2014-03
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.837074
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Contexts</italic>: <italic>Agarum clathratum</italic> (Laminariaceae), a typical brown algae, has been identified by National Plant Quarantine Service in Korea. The extract of <italic>A. clathratum</italic> has antioxidant activities.</p> <p> <italic>Objective</italic>: We investigated the neuroprotective effects of crude-extract, ethyl acetate (EA)-, <italic>n</italic>-butanol (BU)-, dichloromethane (DCM)- and <italic>n</italic>-hexane (Hx)-fractions from <italic>A. clathratum</italic> on ischemic damage in the gerbil hippocampal CA1 region (CA1) after 5 min of transient cerebral ischemia.</p> <p> <italic>Materials and methods</italic>: <italic>Agarum clathratum</italic> was collected in Kangwon province (South Korea) and treated with 95% ethanol. The ethanol extract was suspended in distilled water and subjected to a series of partitions with EA, BU, DCM and Hx. Each of extract and fraction was orally administered with 50 mg/kg once a day for one week before ischemia--reperfusion (I-R).</p> <p> <italic>Result</italic>: In the crude-extract-, EA- and BU-fraction-treated ischemia groups, we found strong neuroprotection in the CA1 – about 80–89% of CA1 pyramidal neurons survived. However, in the DCM- and Hx-fraction-treated ischemia groups, we did not find any significant neuroprotection. In addition, we observed changes in astrocytes and microglia in the ischemic CA1. In the crude-extract, EA- and BU-fraction-treated ischemia groups, the distribution pattern and activity of the glial cells were similar to that found in the sham group.</p> <p> <italic>Discussion</italic>: Repeated supplements of crude-extract, EA- and BU-fractions of <italic>A. clathratum</italic> could protect neurons from I-R injury in the hippocampal CA1 induced by transient cerebral ischemia via decrease of glial activation.</p> </abstract>