TALEN‐induced gene knock out in Drosophila. (31st October 2013)

• Record Type: Journal Article
• Authors: Kondo, Takefumi; Sakuma, Tetsushi; Wada, Housei; Akimoto‐Kato, Ai; Yamamoto, Takashi; Hayashi, Shigeo
• Is Part Of: Development growth and differentiation. Volume 56:Number 1(2014)
• Page Start: 86
• Page End: 91
• Publication Date: 2013-10-31
• Subjects: Embryology -- Periodicals; Developmental biology -- Periodicals; Growth -- Periodicals; 574.3
• DOI: 10.1111/dgd.12097
• Languages: English
• ISSNs: 0012-1592

Abstract

<abstract abstract-type="main" id="dgd12097-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>We report here a case study of TALEN‐induced gene knock out of the <italic>trachealess</italic> gene of <italic>Drosophila</italic>. Two pairs of TALEN constructs caused targeted mutation in the germ line of 39% and 17% of injected animals, respectively. In the extreme case 100% of the progeny of TALEN‐injected fly was mutated, suggesting that highly efficient biallelic germ line mutagenesis was achieved. The mutagenic efficiency of the TALEN pairs paralleled their activity of single strand annealing (SSA) assay in cultured cells. All mutations were deletion of 1 to 20 base pairs. Merit and demerit of TALEN‐based gene knockout approach compared to other genome editing technologies is discussed.</p> </abstract>