Complete sequence analysis of two cryptic plasmids from Bifidobacterium kashiwanohense JCM 15439 (type strain) isolated from healthy infant feces. (19th July 2013)

• Record Type: Journal Article
• Authors: Takahata, Muneaki; Toh, Hidehiro; Nakano, Akiyo; Takagi, Misako; Murakami, Masaru; Ishii, Yasuo; Takizawa, Tatsuya; Tanabe, Soichi; Morita, Hidetoshi
• Is Part Of: Animal science journal. Volume 85:Number 2(2014:Feb.)
• Page Start: 158
• Page End: 163
• Publication Date: 2013-07-19
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12095
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Bifidobacterial plasmids reported so far are derived from a limited number of strains and plasmids of bifidobacterial type strains isolated from humans are unknown. We found that <italic>Bifidobacterium kashiwanohense</italic> JCM 15439 (type strain) isolated from a healthy infant contained two cryptic plasmids, designated pBBKW‐1 and pBBKW‐2. We determined and analyzed the complete sequences of both plasmids. pBBKW‐1 (7716 bp) was predicted to replicate by a rolling‐circle mechanism and encode six protein‐coding genes, two of which are putative replication proteins. pBBKW‐1 seems to be a cointegrate plasmid containing two copies of the plasmid pMG1 from <italic>Bifidobacterium longum</italic>. pBBKW‐2 (2920 bp) was predicted to encode six protein‐coding genes and be a theta‐type replicating plasmid, which has been reported to be more stable than a rolling circle‐type replicating plasmid frequently found in bifidobacteria. Our finding will provide new insights into safe recombinant plasmid constructions for humans.</p> </abstract>