Characterisation of the Metabolism of Pogostone In Vitro and In Vivo Using Liquid Chromatography with Mass Spectrometry. Issue 2 (26th November 2013)

• Record Type: Journal Article
• Authors: Li, Yucui; Su, Ziren; Lin, Shuhai; Li, Chuwen; Zhao, Ya; Gao, Xiang; Lai, Yongquan; Wu, Xiaoli; Wu, Hanzhi; Cai, Zongwei; Lai, Xiaoping
• Is Part Of: Phytochemical analysis. Volume 25:Issue 2(2014:Mar.)
• Page Start: 97
• Page End: 105
• Publication Date: 2013-11-26
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2471
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2471-sec-0001" sec-type="section"> <title>Introduction</title> <p>Pogostone possesses potent anti‐bacterial and anti‐fungal activities and has been used for the quality control of essential oil of <italic>Pogostemon cablin</italic>. Pogostone is easily absorbed after oral administration but its metabolism in mammals remains elusive.</p> </sec> <sec id="pca2471-sec-0002" sec-type="section"> <title>Objective</title> <p>To investigate the metabolic profile of pogostone <italic>in vitro</italic> and <italic>in vivo</italic>.</p> </sec> <sec id="pca2471-sec-0003" sec-type="section"> <title>Methods</title> <p>High‐performance liquid chromatography coupled with mass spectrometry (LC–MS) techniques were employed. Orbitrap MS and ion trap tandem mass spectrometry (MS/MS) were utilised to analyse the metabolism of pogostone by virtue of the high sensitivity and high selectivity in the measurement. <italic>In vitro</italic> experiment was carried out using rat liver microsomes while the <italic>in vivo</italic> study was conducted on rats, which were orally administered with pogostone (80 mg/kg).</p> </sec> <sec id="pca2471-sec-0004" sec-type="section"> <title>Results</title> <p>In total, three mono‐hydroxylated, one di‐hydroxylated, one mono‐oxygenated, one di‐oxygenated metabolite, one hydrolysis and one hydroxy conjugated metabolites were found. In addition hydroxylation was demonstrated to be a major metabolic pathway of pogostone.</p> </sec> <sec id="pca2471-sec-0005" sec-type="section"> <title>Conclusion</title> <p>LC–MS was demonstrated to be a powerful tool for the metabolite identification of pogostone. The tentative identification of metabolites provides an insight for the metabolic clues of pogostone. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>