Identification of Nuclear Factor‐κB Inhibitors and β2 Adrenergic Receptor Agonists in Chinese Medicinal Preparation Fuzilizhong Pills Using UPLC with Quadrupole Time‐of‐flight MS. Issue 2 (23rd September 2013)

• Record Type: Journal Article
• Authors: Dong, Linyi; Cheng, Binfeng; Luo, Yi; Zhang, Ning; Duan, Hongquan; Jiang, Min; Wang, Yiming; Bai, Gang; Luo, Guoan
• Is Part Of: Phytochemical analysis. Volume 25:Issue 2(2014:Mar.)
• Page Start: 113
• Page End: 121
• Publication Date: 2013-09-23
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2474
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <sec id="pca2474-sec-0001" sec-type="section"> <title>Introduction</title> <p>Fuzilizhong Pills (FZLZ), a modified form of a famous traditional Chinese medicine (TCM) Lizhong Wan in <italic>Treatise on Febrile Disease</italic>s and consisting of <italic>Panax ginseng</italic> C.A.Mey. (Ren Shen), <italic>Aconitum carmichaelii</italic> Debx. (Fu Zi, Zhi), <italic>Glycyrrhiza uralensis</italic> Fisch., <italic>Glycyrrhiza inflata</italic> Bat. or <italic>Glycyrrhiza glabra</italic> L. (Gan Cao), <italic>Atractylodes macrocephala</italic> Koidz. (Bai Zhu) and <italic>Zingiber offcinale</italic> Rosc. (Gan Jiang), show strong clinical therapeutic effects for dyspnea and pulmonary oedema. However, the bioactive compounds are still unclear. In this study, FZLZ was analysed using a rapid detection method to identify its anti‐inflammatory and spasmolytic constituents.</p> </sec> <sec id="pca2474-sec-0002" sec-type="section"> <title>Objective</title> <p>To develop a simple screening method to detect the anti‐inflammatory and spasmolytic constituents of FZLZ.</p> </sec> <sec id="pca2474-sec-0003" sec-type="section"> <title>Methods</title> <p>Ultra‐performance liquid chromatography with quadrupole time‐of‐flight mass spectrometry combined with dual‐bioactive (NF‐κB and β<sub>2</sub>‐adrenergic receptor) luciferase reporter assay systems was employed.</p> </sec> <sec id="pca2474-sec-0004" sec-type="section"> <title>Results</title> <p>Two β<sub>2</sub>‐adrenergic receptor agonists (salsolinol and higeramine) and three terpenoidal analogues of NF‐κB inhibitors such as ginsenosides derivatives, triperpenoids derivatives and diester‐diterpenoid aconitum alkaloid derivatives were characterised. Mesaconitine, flaconitine, ginsenosides Rb2, Rf, Rg2, F1 and Ro were considered to be new NF‐κB inhibitors. Furthermore, IL‐8 detection by enzyme linked immunosorbent assay confirmed the anti‐inflammatory effects of the potential NF‐κB inhibitors.</p> </sec> <sec id="pca2474-sec-0005" sec-type="section"> <title>Conclusion</title> <p>Compared with conventional fingerprints, activity‐integrated fingerprints that contain both chemical and bioactive details offer a more comprehensive understanding of the chemical composition of plant materials. This strategy clearly demonstrated that dual bioactivity‐integrated fingerprinting is a powerful tool for the improved screening and identification of potential dual‐target lead compounds in complex herbal medicines. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>