DNA barcodes and molecular diagnostics for distinguishing introduced Xyleborus (Coleoptera: Scolytinae) species in China. (February 2014)

• Record Type: Journal Article
• Authors: Chang, Hong; Liu, Qun; Hao, Dejun; Liu, Yong; An, Yulin; Qian, Lu; Yang, Xiaojun
• Is Part Of: Mitochondrial DNA. Volume 25:Number 1(2014:Feb.)
• Page Start: 63
• Page End: 69
• Publication Date: 2014-02
• Subjects: Mitochondrial DNA -- Periodicals; Mitochondrial DNA -- Abnormalities -- Periodicals; Nucleotide sequence -- Periodicals; Gene mapping -- Periodicals; 572.869
• DOI: 10.3109/19401736.2013.779260
• Languages: English
• ISSNs: 1940-1736

Abstract

<abstract> <title>Abstract</title> <p>Members of the large and complex genus <italic>Xyleborus</italic> (Coleoptera: Scolytinae: Xyleborini) are the most commonly intercepted beetles associated with solid wood-packing materials at ports of entry in China. The accurate identification of species is critical in preventing the invasion of exotic insects. Considering the difficulties in morphological identification, genetic divergences of mitochondrial cytochrome <italic>c</italic> oxidase subunit I (COI) genes have been used in insect species identification. In this study, 32 <italic>Xyleborus</italic> and 2 outgroup species were collected from Jiangsu ports and selected to evaluate the effectiveness of DNA barcoding for <italic>Xyleborus</italic> species. The results showed that the mean interspecific divergence values (23.6%) were 15-fold higher than the observed intraspecific divergence (1.6%), except <italic>Xyleborus affinis</italic>. The results supported the inference that the barcode variation within species of insects is somewhat higher than interspecific ones. Thus, this study validated the effectiveness of barcoding for the identification of <italic>Xyleborus</italic> species.</p> </abstract>