Hypertonic HBOC-201 Decreases Neutrophil Activation after Hemorrhagic Shock. (1st February 2014)

• Record Type: Journal Article
• Authors: Rivera-Chávez, Fernando A.; Lu, Amanda; Liu, Ming-Mei; Abdalla, Adil; Minei, Joseph P.
• Is Part Of: Journal of investigative surgery. Volume 27:Number 1(2014:Feb.)
• Page Start: 14
• Page End: 20
• Publication Date: 2014-02-01
• Subjects: Surgery -- Research -- Periodicals; Research; Surgery; Surgical Procedures, Operative; 617.075
• DOI: 10.3109/08941939.2013.826756
• Languages: English
• ISSNs: 0894-1939

Abstract

<abstract> <title>ABSTRACT</title> <p> <italic>Objective</italic>: To evaluate neutrophil activation after exposure to standard HBC-201 (suspended in lactate Ringer's solution) versus HBOC-201 suspended in hypertonic 7.5% saline solution. <italic>Methods</italic>: We use plasma and tissue obtained from pigs subjected to controlled hemorrhagic shock and an ex vivo model of stimulated human whole blood. The pigs were resuscitated with the following (<italic>n</italic> = 8 per group) standard HBOC-201, or hypertonic HBOC-201. We used HTS 7.5%, Ringer's lactate as control resuscitation. Human blood was stimulated with same fluids. We measured the following neutrophil markers; IL-8, H<sub>2</sub>O<sub>2</sub> in pig plasma, MPO in pig tissue, and H<sub>2</sub>O<sub>2</sub>, IL-8, and CD11b/CD18 in human whole blood. <italic>Results</italic>: H<sub>2</sub>O<sub>2</sub> and IL-8 as well as tissue MPO were significantly decreased in pigs resuscitated with HT-HBOC-201 and HT 7.5%. Ex vivo experiments blood diluted with HTS and HT-HBOC-201 revealed lower expression of CD11b/CD18, H<sub>2</sub>O<sub>2</sub>, and IL-8. Blood diluted with HBOC-201 had a higher CD11b/CD18 expression than blood diluted with LR solution. <italic>Conclusion</italic>: Our in vivo and ex vivo experiments indicate that HBOC-201 suspended in hypertonic 7.5% saline solution is associated with significantly less neutrophil activation when compared to standard HBOC-201 suspended in lactate Ringer's solution.</p> </abstract>