Allelic polymorphism and inheritance of MdACS1 and MdACO1 genes in apple (Malus × domestica Borkh.). Issue 1 (5th December 2013)

• Record Type: Journal Article
• Authors: Marić, Slađana; Lukić, Milan; Flachowsky, H.
• Is Part Of: Plant breeding. Volume 133:Issue 1(2014)
• Page Start: 108
• Page End: 114
• Publication Date: 2013-12-05
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12124
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12124-abs-0001"> <title>Abstract</title> <p>The allelic polymorphism and inheritance of <italic>MdACS1</italic> and <italic>MdACO1</italic> genes were analysed in 28 apple cultivars, which were derived from reciprocal crosses of the following parental pairs: 'Golden Delicious' × 'James Grieve', 'Golden Delicious' × 'Jonathan', 'Cox's Orange Pippin' × 'Golden Delicious', 'Cox's Orange Pippin' × 'Jonathan'. Polymorphisms were detected by PCR and the use of two restriction enzymes (<italic>Bam</italic>H1 and <italic>Rsa</italic>I). In addition, new primers were designed for the further discrimination of <italic>MdACO1</italic> alleles. Two alleles of <italic>MdACS1</italic> gene (<italic>MdACS1‐1</italic> and <italic>MdACS1‐2</italic>) and three alleles of <italic>MdACO1</italic> gene (<italic>a</italic>, <italic> b</italic> and <italic>c</italic>) were detected. Cloning and sequencing of <italic>MdACO1</italic> alleles confirmed a high conservation and some differences within the coding regions and helped to reconcile between different numbering systems. Observed segregations confirmed that alleles <italic>a</italic>, <italic> b</italic> and <italic>c</italic> belonged to the <italic>MdACO1</italic> gene. It is apparent that polymorphisms within the <italic>MdACS1</italic> and <italic>MdACO1</italic> genes could aid cultivar genotyping and identification and, furthermore, that the <italic>MdACS1‐2/2</italic> genotype is correlated with a long apple storage life.</p> </abstract>