An efficient reverse genetics platform in the model legume Medicago truncatula. Issue 3 (11th November 2013)

• Record Type: Journal Article
• Authors: Cheng, Xiaofei; Wang, Mingyi; Lee, Hee‐Kyung; Tadege, Million; Ratet, Pascal; Udvardi, Michael; Mysore, Kirankumar S.; Wen, Jiangqi
• Is Part Of: New phytologist. Volume 201:Issue 3(2014)
• Page Start: 1065
• Page End: 1076
• Publication Date: 2013-11-11
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12575
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12575-abs-0001"> <title>Summary</title> <p> <list id="nph12575-list-0001" list-type="bullet"> <list-item> <p> <italic>Medicago truncatula</italic> is one of the model species for legume studies. In an effort to develop legume genetics resources, > 21 700 <italic>Tnt1</italic> retrotransposon insertion lines have been generated.</p> </list-item> <list-item> <p>To facilitate fast‐growing needs in functional genomics, two reverse genetics approaches have been established: web‐based database searching and PCR‐based reverse screening. More than 840 genes have been reverse screened using the PCR‐based approach over the past 6 yr to identify mutants in these genes. Overall, <italic>c</italic>. 84% (705 genes) success rate was achieved in identifying mutants with at least one <italic>Tnt1</italic> insertion, of which <italic>c</italic>. 50% (358 genes) had three or more alleles.</p> </list-item> <list-item> <p>To demonstrate the utility of the two reverse genetics platforms, two mutant alleles were isolated for each of the two floral homeotic MADS‐box genes, <italic>MtPISTILATA</italic> and <italic>MtAGAMOUS</italic>. Molecular and genetic analyses indicate that <italic>Tnt1</italic> insertions in exons of both genes are responsible for the defects in floral organ development.</p> </list-item> <list-item> <p>In summary, we have developed two efficient reverse genetics platforms to facilitate functional characterization of <italic>M. truncatula</italic> genes.</p> </list-item> </list> </p> </abstract>