The effect of IL‐17 on the production of proinflammatory cytokines and matrix metalloproteinase‐1 by human periodontal ligament fibroblasts. (February 2014)

• Record Type: Journal Article
• Authors: Shibata, M.; Shintaku, Y.; Matsuzaki, K.; Uematsu, S.
• Is Part Of: Orthodontics and craniofacial research. Volume 17:Number 1(2014:Feb.)
• Page Start: 60
• Page End: 68
• Publication Date: 2014-02
• Subjects: Skull -- Growth -- Periodicals; Facial bones -- Growth -- Periodicals; Orthodontics -- Periodicals; Orthodontics, Corrective -- Periodicals; Orthodontic appliances -- Periodicals; 617.51
• DOI: 10.1111/ocr.12033
• Languages: English
• ISSNs: 1601-6335

Abstract

<abstract abstract-type="main" id="ocr12033-abs-0001"> <title>Structured Abstract</title> <sec id="ocr12033-sec-0001" sec-type="section"> <title>Objectives</title> <p>To investigate the effects of IL‐17 on IL‐6, IL‐1β, and matrix metalloproteinase (MMP‐1) production, and to compare the MMP‐1 production between the individual and combined effects of IL‐1β and IL‐6 in human periodontal ligament fibroblasts (HPDLF).</p> </sec> <sec id="ocr12033-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Human periodontal ligament fibroblasts were cultured with IL‐17 for 0.5, 1, 4, 24, 48, and 72 h, and were cultured with IL‐1β, IL‐6/sIL‐6R, or a combination of IL‐1β and IL‐6/sIL‐6R for 24 h. To measure the mRNA levels of <italic>IL‐6</italic>, <italic> IL‐1β, </italic> and <italic>MMP‐1</italic>, total RNA was extracted from the cultured HPDLF, and a real‐time PCR analysis was performed. The protein levels of IL‐6, IL‐1β, and MMP‐1 in supernatants were measured using enzyme‐linked immunosorbent assays (ELISAs).</p> </sec> <sec id="ocr12033-sec-0003" sec-type="section"> <title>Results</title> <p>IL‐17 significantly increased the expression of <italic>IL‐6</italic> and <italic>MMP‐1</italic> mRNA and protein, while IL‐17 transiently increased the expression of <italic>IL‐1β</italic> mRNA. The combination of IL‐1β and IL‐6/sIL‐6R induced significantly higher levels of MMP‐1 protein than IL‐1β alone.</p> </sec> <sec id="ocr12033-sec-0004" sec-type="section"> <title>Conclusions</title> <p>IL‐17 upregulated the production of IL‐6 and MMP‐1 sequentially in HPDLF. IL‐6/sIL‐6R may enhance the effects of IL‐1β on MMP‐1 production. The present results suggest that IL‐17 induces MMP‐1 production not only directly, but also indirectly by promoting IL‐6 production, thus resulting in the degradation of collagens in the PDL.</p> </sec> </abstract>