A challenge with Porphyromonas gingivalis differentially affects the osteoclastogenesis potential of periodontal ligament fibroblasts from periodontitis patients and non‐periodontitis donors. (19th November 2013)

• Record Type: Journal Article
• Authors: Sokos, Dimitris; Scheres, Nina; Schoenmaker, Ton; Everts, Vincent; de, Teun J.
• Is Part Of: Journal of clinical periodontology. Volume 41:Number 2(2014:Feb.)
• Page Start: 95
• Page End: 103
• Publication Date: 2013-11-19
• Subjects: Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.12186
• Languages: English
• ISSNs: 0303-6979

Abstract

<abstract abstract-type="main" id="jcpe12186-abs-0001"> <title>Abstract</title> <sec id="jcpe12186-sec-0001" sec-type="section"> <title>Aim</title> <p> <italic>Porphyromonas gingivalis</italic> (<italic>Pg</italic>) may cause an immune‐inflammatory response in host cells leading to bone degradation by osteoclasts. We investigated the osteoclast‐inducing capacity of periodontal ligament fibroblasts from periodontitis patients and non‐periodontitis donors after a challenge with viable <italic>Pg</italic>.</p> </sec> <sec id="jcpe12186-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>PDLFs from periodontitis patients (<italic>n</italic> = 8) and non‐periodontitis donors (<italic>n</italic> = 7) were incubated for 6 h with or without viable <italic>Pg</italic> and subsequently co‐cultured with osteoclast precursors from peripheral blood mononuclear cells (PBMCs). The number of multinucleated tartrate‐resistant acid phosphatase‐positive cells was determined at 21 days. Expression of osteoclastogenesis‐associated genes was assessed after infection of PDLFs mono‐cultures and in PDLFs‐PBMCs co‐cultures. Resorption activity was analysed on bone slices.</p> </sec> <sec id="jcpe12186-sec-0003" sec-type="section"> <title>Results</title> <p> <italic>Pg</italic> induced the expression of osteoclastogenesis‐associated genes by PDLFs. After bacterial challenge the formation of osteoclast‐like cell was decreased in co‐cultures of PBMCs with non‐periodontitis PDLFs, but not with PDLFs from periodontitis patients.</p> </sec> <sec id="jcpe12186-sec-0004" sec-type="section"> <title>Conclusion</title> <p>PDLFs from sites free of periodontitis respond to an infection with <italic>Pg</italic> by tempering formation of osteoclast‐like cells, probably promoting clearance of the infection. PDLFs from periodontitis sites are desensitized to a <italic>Pg</italic> challenge in terms of their osteoclast‐inducing capacity.</p> </sec> </abstract>