The expression of prostaglandin receptors EP3 and EP4 in human cervix in post‐term pregnancy differs between failed and successful labor induction. (18th December 2013)

• Record Type: Journal Article
• Authors: Roos, Nathalie; Blesson, Chellakkan S.; Stephansson, Olof; Masironi, Britt; Vladic Stjernholm, Ylva; Ekman‐Ordeberg, Gunvor; Sahlin, Lena
• Is Part Of: Acta obstetricia et gynecologica Scandinavica. Volume 93:Number 2(2014)
• Page Start: 159
• Page End: 167
• Publication Date: 2013-12-18
• Subjects: Gynecology -- Periodicals; Pregnancy -- Periodicals; Obstetrics -- Periodicals; 618.05
• DOI: 10.1111/aogs.12300
• Languages: English
• ISSNs: 0001-6349

Abstract

<abstract abstract-type="main" id="aogs12300-abs-0001"> <title>Abstract</title> <sec id="aogs12300-sec-0001" sec-type="section"> <title>Objective</title> <p>To investigate expression and localization of prostaglandin receptors EP1‐4 and FP and localization of stromal factors CTGF (connective tissue growth factor), furin, calgranulin B and ALOX15 (arachidonate 15‐lipooxygenase) in human cervical tissue from post‐term women with failed or successful labor induction after prostaglandin priming.</p> </sec> <sec id="aogs12300-sec-0002" sec-type="section"> <title>Design</title> <p>Experimental prospective clinical study.</p> </sec> <sec id="aogs12300-sec-0003" sec-type="section"> <title>Setting</title> <p>Tertiary obstetric care center.</p> </sec> <sec id="aogs12300-sec-0004" sec-type="section"> <title>Population</title> <p>Twenty‐six women giving birth post‐term, with failed or successful labor induction, and a control group consisting of 19 women with spontaneous onset of labor and delivery at term.</p> </sec> <sec id="aogs12300-sec-0005" sec-type="section"> <title>Methods</title> <p>Biopsies were obtained from post‐term women with successful (responders; R) and failed (non‐responders; NR) labor induction. Women with spontaneous delivery at term were included as controls (C). mRNA expression was determined with real time PCR, protein expression and localization with immunohistochemistry.</p> </sec> <sec id="aogs12300-sec-0006" sec-type="section"> <title>Main outcome measures</title> <p>Comparisons of mRNA and protein expressions between post‐term pregnancies with failed and successful labor induction as well as term controls.</p> </sec> <sec id="aogs12300-sec-0007" sec-type="section"> <title>Results</title> <p>EP4 mRNA expression was down‐regulated concomitant with an up‐regulation of EP3 mRNA expression in cervix from the NR group as compared with the R group. In stroma, immunoreactivity of the EP4 protein was increased in the NR group as compared with R and C groups.</p> </sec> <sec id="aogs12300-sec-0008" sec-type="section"> <title>Conclusions</title> <p>Failure of cervical ripening, after local application of prostaglandins for labor induction, may be caused by the increased expression of EP3 and concomitant decrease in EP4 expression.</p> </sec> </abstract>