Protein biomarkers in vernix with potential to predict the development of atopic eczema in early childhood. Issue 1 (11th November 2013)

• Record Type: Journal Article
• Authors: Holm, T.; Rutishauser, D.; Kai‐Larsen, Y.; Lyutvinskiy, Y.; Stenius, F.; Zubarev, R. A.; Agerberth, B.; Alm, J.; Scheynius, A.
• Is Part Of: Allergy. Volume 69:Issue 1(2014:Jan.)
• Page Start: 104
• Page End: 112
• Publication Date: 2013-11-11
• Subjects: Allergy -- Periodicals; 616.97
• DOI: 10.1111/all.12308
• Languages: English
• ISSNs: 0105-4538

Abstract

<abstract abstract-type="main" id="all12308-abs-0001"> <title>Abstract</title> <sec id="all12308-sec-0001" sec-type="section"> <title>Background</title> <p>Atopic eczema (AE) is a chronic inflammatory skin disease, which has increased in prevalence. Evidence points toward lifestyle as a major risk factor. AE is often the first symptom early in life later followed by food allergy, asthma, and allergic rhinitis. Thus, there is a great need to find early, preferentially noninvasive, biomarkers to identify individuals that are predisposed to AE with the goal to prevent disease development.</p> </sec> <sec id="all12308-sec-0002" sec-type="section"> <title>Objective</title> <p>To investigate whether the protein abundances in vernix can predict later development of AE.</p> </sec> <sec id="all12308-sec-0003" sec-type="section"> <title>Methods</title> <p>Vernix collected at birth from 34 newborns within the Assessment of Lifestyle and Allergic Disease During INfancy (ALADDIN) birth cohort was included in the study. At 2 years of age, 18 children had developed AE. Vernix proteins were identified and quantified with liquid chromatography coupled to tandem mass spectrometry.</p> </sec> <sec id="all12308-sec-0004" sec-type="section"> <title>Results</title> <p>We identified and quantified 203 proteins in all vernix samples. An orthogonal projections to latent structures‐discriminant analysis (OPLS‐DA) model was found with <italic>R</italic><sup>2</sup> = 0.85, <italic>Q</italic><sup>2</sup> = 0.39, and discrimination power between the AE and healthy group of 73.5%. Polyubiquitin‐C and calmodulin‐like protein 5 showed strong negative correlation to the AE group, with a correlation coefficient of 0.73 and 0.68, respectively, and a <italic>P</italic>‐value of 8.2 E‐7 and 1.8 E‐5, respectively. For these two proteins, the OPLS‐DA model showed a prediction accuracy of 91.2%.</p> </sec> <sec id="all12308-sec-0005" sec-type="section"> <title>Conclusion</title> <p>The protein abundances in vernix, and particularly that of polyubiquitin‐C and calmodulin‐like protein 5, are promising candidates as biomarkers for the identification of newborns predisposed to develop AE.</p> </sec> </abstract>