Mitochondrial energetics is impaired in vivo in aged skeletal muscle. Issue 1 (19th September 2013)

• Record Type: Journal Article
• Authors: Gouspillou, Gilles; Bourdel‐Marchasson, Isabelle; Rouland, Richard; Calmettes, Guillaume; Biran, Marc; Deschodt‐Arsac, Véronique; Miraux, Sylvain; Thiaudiere, Eric; Pasdois, Philippe; Detaille, Dominique; Franconi, Jean‐Michel; Babot, Marion; Trézéguet, Véronique; Arsac, Laurent; Diolez, Philippe
• Is Part Of: Aging cell. Volume 13:Issue 1(2014:Feb.)
• Page Start: 39
• Page End: 48
• Publication Date: 2013-09-19
• Subjects: Cells -- Aging -- Periodicals; 571.8783605
• DOI: 10.1111/acel.12147
• Languages: English
• ISSNs: 1474-9718

Abstract

<abstract abstract-type="main" id="acel12147-abs-0001"> <title>Summary</title> <p>With aging, most skeletal muscles undergo a progressive loss of mass and strength, a process termed sarcopenia. Aging‐related defects in mitochondrial energetics have been proposed to be causally involved in sarcopenia. However, changes in muscle mitochondrial oxidative phosphorylation with aging remain a highly controversial issue, creating a pressing need for integrative approaches to determine whether mitochondrial bioenergetics are impaired in aged skeletal muscle. To address this issue, mitochondrial bioenergetics was first investigated <italic>in vivo</italic> in the <italic>gastrocnemius</italic> muscle of adult (6 months) and aged (21 months) male Wistar rats by combining a modular control analysis approach with <sup>31</sup>P magnetic resonance spectroscopy measurements of energetic metabolites. Using this innovative approach, we revealed that the <italic>in vivo</italic> responsiveness ('elasticity') of mitochondrial oxidative phosphorylation to contraction‐induced increase in ATP demand is significantly reduced in aged skeletal muscle, a reduction especially pronounced under low contractile activities. In line with this <italic>in vivo</italic> aging‐related defect in mitochondrial energetics, we found that the mitochondrial affinity for ADP is significantly decreased in mitochondria isolated from aged skeletal muscle. Collectively, the results of this study demonstrate that mitochondrial bioenergetics are effectively altered <italic>in vivo</italic> in aged skeletal muscle and provide a novel cellular basis for this phenomenon.</p> </abstract>