Discrimination between invasive Ponto‐Caspian gobies using a PCR‐RFLP method. (26th August 2013)

• Record Type: Journal Article
• Authors: Kohout, J.; Pekárik, L.; Šedivá, A.; Didenko, A.; Čiampor, F.; Čiamporová‐Zaťovičová, Z.
• Is Part Of: Journal of applied ichthyology. Volume 30:Number 1(2014:Feb.)
• Page Start: 121
• Page End: 126
• Publication Date: 2013-08-26
• Subjects: Fishes -- Periodicals; Fish culture -- Periodicals; Fisheries -- Periodicals; Ichthyology -- Periodicals; 597
• DOI: 10.1111/jai.12315
• Languages: English
• ISSNs: 0175-8659

Abstract

<abstract abstract-type="main" id="jai12315-abs-0001"> <title>Summary</title> <p>Accurate identification of invaders, and especially their juveniles and eggs, is a difficult task if several morphologically similar species co‐occur. The aim of the study was to develop and test a rapid and cost‐effective procedure for identification of five species of invasive gobies occurring in the middle Danube basin, namely round goby <italic>Neogobius melanostomus</italic>, bighead goby <italic>Ponticola kessleri</italic>, monkey goby <italic>Neogobius fluviatilis</italic>, racer goby <italic>Babka gymnotrachelus</italic> and tubenose goby <italic>Proterorhinus semilunaris</italic>. First, a 708 bp fragment of the cytochrome oxidase I gene was amplified and sequenced for representative samples of these five species. Appropriate sequences of the five species available in public databases were used for <italic>in silico</italic> analysis. A digestion of the amplified fragment with the <italic>Bfa</italic>I enzyme was found to be suitable for the species identification, as it showed unique restriction patterns for each species. The technique was also successfully applied for fish remains from burbot <italic>Lota lota</italic> stomachs. Thus the technique could be a useful tool in monitoring biological invasions, especially by identifying specimens that could not be determined on the basis of morphological features. The results demonstrate that the PCR‐RFLP method may in some cases be more reliable for species identification than a standard DNA sequencing.</p> </abstract>