Clinical and molecular characterization of Hb Hofu in eastern India. (25th July 2013)

• Record Type: Journal Article
• Authors: Purohit, P.; Mashon, R. S.; Patel, S.; Dehury, S.; Pattanayak, C.; Das, K.; Nair, S.; Italia, K.; Bag, S.; Colah, R.; Patel, D. K.
• Is Part Of: International journal of laboratory hematology. Volume 36:Number 1(2014:Feb.)
• Page Start: 71
• Page End: 76
• Publication Date: 2013-07-25
• Subjects: Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.12128
• Languages: English
• ISSNs: 1751-5521

Abstract

<abstract abstract-type="main" id="ijlh12128-abs-0001"> <title>Summary</title> <sec id="ijlh12128-sec-0001" sec-type="section"> <title>Introduction</title> <p>Hb Hofu (HBB:c. 380T>A) is a rare inherited hemoglobin abnormality with few case reports in the world literature.</p> </sec> <sec id="ijlh12128-sec-0002" sec-type="section"> <title>Methods</title> <p>Screening for the sickle cell gene mutation and other hemoglobinopathies was carried out using the sickle slide test, Hb electrophoresis, and HPLC under an ongoing central government project.</p> </sec> <sec id="ijlh12128-sec-0003" sec-type="section"> <title>Results</title> <p>We detected twelve Hb Hofu heterozygotes and three sickle Hb Hofu compound heterozygotes. The heterozygotes were asymptomatic except for one individual who had chronic kidney disease and moderate anemia. Only one HbS‐Hofu case was symptomatic and presented with intermittent attacks of painful crisis. In the carrier state, the Hb Hofu eluted as a hump at the beginning of the HbA<sub>0</sub> window. But in HbS‐Hofu cases, Hb Hofu eluted as a single peak in the HbA<sub>0</sub> window, with the HbA<sub>2</sub> levels being >4% consistently.</p> </sec> <sec id="ijlh12128-sec-0004" sec-type="section"> <title>Conclusion</title> <p>HbS‐Hofu has a variable clinical presentation. The retention time of Hb Hofu on HPLC is very close to that of HbA<sub>0</sub> and often elutes in the A<sub>0</sub> window. Thus, there is every possibility of the HbS‐Hofu chromatogram to be misinterpreted as that of a sickle cell trait/transfused sickle cell‐beta‐thalassemia case. This is the first time where Hb Hofu has been detected by HPLC, which is the widely accepted screening technique for hemoglobinopathies around the world.</p> </sec> </abstract>