Expression of Podocalyxin Separates the Hematopoietic and Vascular Potentials of Mouse Embryonic Stem Cell‐Derived Mesoderm. (January 2014)

• Record Type: Journal Article
• Authors: Zhang, Hailan; Nieves, Johnathan L.; Fraser, Stuart T.; Isern, Joan; Douvaras, Panagiotis; Papatsenko, Dmitri; D'Souza, Sunita L.; Lemischka, Ihor R.; Dyer, Michael A.; Baron, Margaret H.
• Is Part Of: Stem cells. Volume 32:Number 1(2014:Jan.)
• Page Start: 191
• Page End: 203
• Publication Date: 2014-01
• Subjects: Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.1536
• Languages: English
• ISSNs: 1066-5099

Abstract

<abstract abstract-type="main"> <title>A<sc>bstract</sc></title> <p>In the mouse embryo and differentiating embryonic stem cells, the hematopoietic, endothelial, and cardiomyocyte lineages are derived from Flk1+ mesodermal progenitors. Here, we report that surface expression of Podocalyxin (Podxl), a member of the CD34 family of sialomucins, can be used to subdivide the Flk1+ cells in differentiating embryoid bodies at day 4.75 into populations that develop into distinct mesodermal lineages. Definitive hematopoietic potential was restricted to the Flk1+Podxl+ population, while the Flk1‐negative Podxl+ population displayed only primitive erythroid potential. The Flk1+Podxl‐negative population contained endothelial cells and cardiomyocyte potential. Podxl expression distinguishes Flk1+ mesoderm populations in mouse embryos at days 7.5, 8.5, and 9.5 and is a marker of progenitor stage primitive erythroblasts. These findings identify Podxl as a useful tool for separating distinct mesodermal lineages. S<sc>tem</sc> C<sc>ells</sc><italic>2014;32:191–203</italic></p> </abstract>