CpG methylation of dmrt1 and cyp19a promoters in relation to their sexual dimorphic expression in the Japanese flounder Paralichthys olivaceus. Issue 1 (23rd December 2013)

• Record Type: Journal Article
• Authors: Wen, A. Y.; You, F.; Sun, P.; Li, J.; Xu, D. D.; Wu, Z. H.; Ma, D. Y.; Zhang, P. J.
• Is Part Of: Journal of fish biology. Volume 84:Issue 1(2014)
• Page Start: 193
• Page End: 205
• Publication Date: 2013-12-23
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12277
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12277-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12277-para-0001">To better understand the effects of DNA methylation on the expression patterns of <italic>dmrt1</italic> (Doublesex and Mab‐3‐related transcription factor 1) and <italic>cyp19a</italic> (Cytochrome P450 19a) in the Japanese flounder <italic>Paralichthys olivaceus</italic>, quantitative expressions, cellular distributions and cytosine‐p‐guanine (CpG) methylation patterns of these two genes in the gonads were analysed. The results showed that <italic>P. olivaceus dmrt1</italic> expression was 70 times higher in the testis than in the ovary (<italic>P</italic> < 0·05). Its mRNA was detected clearly in spermatocytes and Sertoli cells of the testis, but weakly in the ovary. <italic>Paralichthys olivaceus cyp19a</italic> expression was 40 times higher in the ovary than in the testis (<italic>P</italic> < 0·01). Its mRNA was detected clearly in follicular cells of the ovary, but weakly in spermatocytes of the testis. The <italic>dmrt1</italic> promoter CpGs were not methylated in the testis, whereas 57·69% were methylated in the ovary. For the <italic>cyp19a</italic> promoter CpGs, 97·5% were methylated in the testis and 73·33% were methylated in the ovary. These findings demonstrate that <italic>P. olivaceus dmrt1</italic> and <italic>cyp19a</italic> are sex‐related genes with sexual dimorphic expression, CpG methylation levels of the two genes are consistent with their expression quantities, and this epigenetic modification can influence the differential expression of genes in the gonads of <italic>P. olivaceus</italic>.</p> </abstract>