Development of a novel rhesus macaque model with an infectious R5 simian–human immunodeficiency virus encoding HIV‐1 CRF08_BC env. Issue 1 (11th September 2013)

• Record Type: Journal Article
• Authors: Huang, Li; Kusagawa, Shigeru; Zeng, Haiyan; Yang, Guibo; Sun, Binlian; Miura, Tomoyuki; Yang, Rongge
• Is Part Of: Journal of medical primatology. Volume 43:Issue 1(2014)
• Page Start: 11
• Page End: 21
• Publication Date: 2013-09-11
• Subjects: Primates -- Periodicals; Primates -- Diseases -- Periodicals; Medicine, Experimental -- Periodicals; 616.02738
• DOI: 10.1111/jmp.12084
• Languages: English
• ISSNs: 0047-2565

Abstract

<abstract abstract-type="main" id="jmp12084-abs-0001"> <title>Abstract</title> <sec id="jmp12084-sec-0001" sec-type="section"> <title>Background</title> <p>The CRF08_BC strain is one of the most predominant circulating Human immunodeficiency virus type 1 (HIV‐1) strains in the Chinese pandemic. A simian–human immunodeficiency virus (SHIV) encoding HIV‐1 CRF08_BC <italic>env</italic> is highly desirable to evaluate candidate AIDS vaccines in non‐human primates.</p> </sec> <sec id="jmp12084-sec-0002" sec-type="section"> <title>Methods</title> <p>SHIV‐KBQJ‐12, which carries the envelope glycoprotein from QJ001, an infectious molecular clone of HIV‐1 CRF08_BC, was generated. The replication capacity of SHIV‐KBQJ‐12 was determined both in human and rhesus macaque (<italic>Macaca mulatta</italic>) peripheral blood mononuclear cells (PBMCs) and in Chinese rhesus macaques.</p> </sec> <sec id="jmp12084-sec-0003" sec-type="section"> <title>Results</title> <p>SHIV‐KBQJ‐12 replicated efficiently in human and macaque PBMCs and displayed a preference for CCR5 as an entry coreceptor. Productive infection of two macaques by intravenous inoculation with SHIV‐KBQJ‐12 was confirmed.</p> </sec> <sec id="jmp12084-sec-0004" sec-type="section"> <title>Conclusions</title> <p>SHIV‐KBQJ‐12 is an R5‐tropic chimeric virus that can establish productive infection both <italic>in vitro</italic> and <italic>in vivo</italic> in Chinese rhesus macaques and will be useful to assess candidate HIV‐1 CRF08_BC vaccines in China.</p> </sec> </abstract>