Immunodetection of a brown planthopper (Nilaparvata lugens Stål) salivary catalase‐like protein into tissues of rice, Oryza sativa. Issue 1 (25th October 2013)

• Record Type: Journal Article
• Authors: Petrova, A.; Smith, C. M.
• Is Part Of: Insect molecular biology. Volume 23:Issue 1(2014:Feb.)
• Page Start: 13
• Page End: 25
• Publication Date: 2013-10-25
• Subjects: Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12058
• Languages: English
• ISSNs: 0962-1075

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Saliva plays an important role in host plant–phloem‐feeding insect molecular interactions. To better elucidate the role of insect saliva, a series of experiments were conducted to establish if catalase from the salivary glands of the brown planthopper (BPH; <italic>Nilaparvata lugens</italic> Stål) was secreted into rice host plant tissue during feeding. Catalase is the main enzyme that decomposes hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) at high concentrations. H<sub>2</sub>O<sub>2</sub> is a part of the free radicals system that mediates important physiological roles including signalling and defence. Previous studies have suggested that H<sub>2</sub>O<sub>2</sub> is involved in the rice endogenous response to BPH feeding. If, the BPH secretes catalase into host plant tissue this will counter the effects of H<sub>2</sub>O<sub>2</sub>, from detoxification to interfering with plant signalling and defence mechanisms. When BPHs were fed on a hopper‐resistant rice variety for 24 h, catalase activity in the salivary glands increased 3.5‐fold compared with hoppers fed on a susceptible rice variety. Further supporting evidence of the effects of BPH catalase was demonstrated by immunodetection analyses where results from two independent sources: BPH‐infested rice tissue and BPH‐probed artificial diets, suggest that the BPH secretes catalase‐like protein during feeding. The possible physiological roles of BPH‐secreted catalase are discussed.</p> </abstract>