Molecular hydrogen protects human lymphocyte AHH-1 cells against 12C6+ heavy ion radiation. (December 2013)

• Record Type: Journal Article
• Authors: Yang, Yanyong; Gao, Fu; Zhang, Hong; Hunag, Yijuan; Zhang, Pei; Liu, Cong; Li, Bailong; Cai, Jianming
• Is Part Of: International journal of radiation biology. Volume 89:Number 12(2013:Dec.)
• Page Start: 1003
• Page End: 1008
• Publication Date: 2013-12
• Subjects: Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2013.817704
• Languages: English
• ISSNs: 0955-3002

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To investigate the potential protective role of molecular hydrogen (H<sub>2</sub>) against <sup>12</sup>C<sup>6+</sup> heavy ion radiation, which is a major hazard for space travel and has been also widely used in heavy ion radiotherapy.</p> <p> <italic>Materials and methods</italic>: H<sub>2</sub> was dissolved in Roswell Park Memorial Institute (RPMI) 1640 medium under high pressure (0.4 Mpa) to a saturated level by using an apparatus produced by our department. A 2-[6-(4′-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) probe and a 2′, 7′-Dichlorodihydrofluorescein diacetate (H2DCFH-DA) fluorescent dye were used to measure the intracellular reactive oxygen species (ROS) level. Cell apoptosis were determined by double-staining with Annexin V-fluorescein isothiocyanate (Annexin V-FITC) and propidium iodide (PI) as well as a Hoechst 33342 staining method alternatively. Subsequently, cell cycle analysis was performed using a PI staining method and the expression of apoptotic protein was examined by Western blot.</p> <p> <italic>Results</italic>: In this study, we demonstrated H<sub>2</sub> reduced ROS level in Human lymphocyte AHH-1 cells as well as in the radiolysis of water. Our data also showed H<sub>2</sub> attenuated <sup>12</sup>C<sup>6+</sup> radiation- induced cell apoptosis and also alleviated radiation-induced G2/M cell cycle arrest. Heavy ion radiation-induced Caspase 3 activation was also inhibited by H<sub>2</sub> treatment.</p> <p> <italic>Conclusion</italic>: In conclusion, these data showed that H<sub>2</sub> attenuated <sup>12</sup>C<sup>6+</sup> radiation-induced cell apoptosis through reducing the ROS level and modulating apoptotic molecules, thus indicating the potential of H<sub>2</sub> as a safe and effective radioprotectant.</p> </abstract>