Quality assessment of buffy‐coat‐derived leucodepleted platelet concentrates in PAS‐plasma, prepared by the OrbiSac or TACSI automated system. Issue 1 (28th July 2013)

• Record Type: Journal Article
• Authors: Plaza, E. M.; Céspedes, P.; Fernández, H.; Sánchez‐Guiu, M. I.; Egea, J. M.; Vicente, V.; Lozano, M. L.; Rivera, J.
• Is Part Of: Vox sanguinis. Volume 106:Issue 1(2014)
• Page Start: 38
• Page End: 44
• Publication Date: 2013-07-28
• Subjects: Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/vox.12072
• Languages: English
• ISSNs: 0042-9007

Abstract

<abstract abstract-type="main" id="vox12072-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vox12072-sec-0001" sec-type="section"> <title>Background and Objectives</title> <p>Buffy‐coat (BC)‐derived platelet concentrates (PCs) are the predominant product for platelet transfusion in many countries. Two automated systems, OrbiSac and TACSI, have been introduced in blood centres to prepare these PCs, as an alternative to the manual method. We compared the <italic>in vitro</italic> quality of PCs prepared by both methods during standard storage.</p> </sec> <sec id="vox12072-sec-0002" sec-type="section"> <title>Study Design and Methods</title> <p>Twenty primary BC pools were split into two parts, which were processed with OrbiSac and TACSI system to obtain OrbiSac PCs (O‐PCs) and TACSI PCs (T‐PCs), respectively. On days 1, 5 and 7 of standard storage, samples were taken and the following analysed: cell count, metabolic variables, platelet function and content of activation and proinflammatory substances.</p> </sec> <sec id="vox12072-sec-0003" sec-type="section"> <title>Results</title> <p>Both the OrbiSac and TACSI systems produced PCs that meet the standards for platelet products in terms of platelet and leucocyte content. <italic>In vitro</italic> evaluation pointed to the similar preservation of platelet metabolism (pH, glucose, bicarbonate and lactate) in O‐PCs and T‐PCs. Moreover, there were no significant differences between O‐PCs and T‐PCs as regards the hypotonic shock response or in the platelet aggregation profile. The OrbiSac system caused greater platelet activation, which resulted in higher concentrations of sCD62P, RANTES and sCD40L on the day the PCs were prepared.</p> </sec> <sec id="vox12072-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The systems OrbiSac and TACSI can be used to produce buffy‐coat‐derived PCs whose cell content, platelet function and metabolism are similar during standard storage. However, the preparation with the OrbiSac system induces a transient increase in platelet activation and release of proinflammatory substances.</p> </sec> </abstract>