Salivary biomarkers: Relationship between oxidative stress and alveolar bone loss in chronic periodontitis. (January 2014)

• Record Type: Journal Article
• Authors: Miricescu, Daniela; Totan, Alexandra; Calenic, Bogdan; Mocanu, Brandusa; Didilescu, Andreea; Mohora, Maria; Spinu, Tudor; Greabu, Maria
• Is Part Of: Acta odontologica Scandinavica. Volume 72:Number 1(2014)
• Page Start: 42
• Page End: 47
• Publication Date: 2014-01
• Subjects: Dentistry -- Periodicals; 617.6005
• DOI: 10.3109/00016357.2013.795659
• Languages: English
• ISSNs: 0001-6357

Abstract

<abstract> <title>Abstract</title> <p> <bold> <italic>Objectives.</italic> </bold> Oxidative stress is implicated in the pathogenesis of many systemic and oral diseases such as periodontal disease. The main aim of this study is to explore a possible association between salivary markers of OS and alveolar bone loss. <bold><italic>Materials and methods.</italic></bold> The study included 20 patients with chronic periodontitis and 20 controls. Salivary OS biomarkers 8-hidroxy-desoxguanosine (8-HOdG), malondialdehyde (MDA), uric acid, total antioxidant capacity (TAC) and glutathione peroxidase (GPx) were evaluated. Bone loss markers such as C-terminal telopeptide of type I collagen (CTX I), matrix metalloproteinases-8 (MMP-8), osteocalcin and 25-hydroxy vitamin D<sub>3</sub> (25- OH D) were detected in this study. The methods included general biochemical tests and ELISA. <bold><italic>Results.</italic></bold> Salivary 8-OHdG, MDA levels were significantly higher in the chronic periodontitis group compared with controls (<italic>p</italic> < 0.05). Salivary activities for uric acid, TAC and GPx were significantly decreased in patients with chronic periodontitis vs controls (<italic>p</italic> < 0.05). Salivary levels for CTX I, MMP-8, 25-OH D and Osteocalcin were significantly higher in the chronic periodontitis group compared to the controls (<italic>p</italic> < 0.05). A significant positive correlation was observed between salivary levels of MDA and CTX I. Significant negative correlations between uric acid and CTX I and between MMP-8 and uric acid have been found. Significant positive correlations were observed between CTX I, MMP-8, 25-OH D, osteocalcin and clinical parameters of periodontal disease. <bold><italic>Conclusions.</italic></bold> Important oxidative stress associated with alveolar bone loss biomarkers can be detected in saliva of patients with periodontal disease.</p> </abstract>