AtSIA1 AND AtOSA1: two Abc1 proteins involved in oxidative stress responses and iron distribution within chloroplasts. Issue 2 (4th October 2013)

• Record Type: Journal Article
• Authors: Manara, Anna; DalCorso, Giovanni; Leister, Dario; Jahns, Peter; Baldan, Barbara; Furini, Antonella
• Is Part Of: New phytologist. Volume 201:Issue 2(2014)
• Page Start: 452
• Page End: 465
• Publication Date: 2013-10-04
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.12533
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph12533-abs-0001"> <title>Summary</title> <p> <list id="nph12533-list-0001" list-type="bullet"> <list-item> <p>The Abc1 protein kinases are a large family of functionally diverse proteins with multiple roles in the regulation of respiration and oxidative stress tolerance.</p> </list-item> <list-item> <p>A functional characterization was carried out for AtSIA1, an <italic>Arabidopsis thaliana</italic> Abc1‐like protein, focusing on its potential redundancy with its homolog AtOSA1. Both proteins are located within chloroplasts, even if a different subplastidial localization seems probable. The comparison of <italic>atsia1</italic> and <italic>atosa1</italic> mutants, <italic>atsia1/atosa1</italic> double mutant and wild‐type plants revealed a reduction in plastidial iron‐containing proteins of the Cyt<italic>b</italic><sub><italic>6</italic></sub><italic>f</italic> complex in the mutants. Iron uptake from soil is not hampered in mutant lines, suggesting that AtSIA1 and AtOSA1 affect iron distribution within the chloroplast.</p> </list-item> <list-item> <p>Mutants accumulated more ferritin and superoxide, and showed reduced tolerance to reactive oxygen species (ROS), potentially indicating a basal role in oxidative stress. The mutants produced higher concentrations of plastochromanol and plastoquinones than wild‐type plants, but only <italic>atsia1</italic> plants developed larger plastoglobules and contained higher concentrations of α‐ and γ‐tocopherol and VTE1.</p> </list-item> <list-item> <p>Taken together, these data suggest that AtSIA1 and AtOSA1 probably act in signaling pathways that influence responses to ROS production and oxidative stress.</p> </list-item> </list> </p> </abstract>