Osteoblastic and cytokine gene expression of implant‐adherent cells in humans. Issue 1 (11th October 2012)

• Record Type: Journal Article
• Authors: Bryington, Matthew; Mendonça, Gustavo; Nares, Salvador; Cooper, Lyndon F.
• Is Part Of: Clinical oral implants research. Volume 25:Issue 1(2014:Jan.)
• Page Start: 52
• Page End: 58
• Publication Date: 2012-10-11
• Subjects: Dental implants -- Research -- Periodicals; 617.69
• DOI: 10.1111/clr.12054
• Languages: English
• ISSNs: 0905-7161

Abstract

<abstract abstract-type="main" id="clr12054-abs-0001"> <title>Abstract</title> <sec id="clr12054-sec-0001" sec-type="section"> <title>Objectives</title> <p>Implant surface topography is a key determinant affecting osteoblastic differentiation and cell–cell signaling of implant‐adherent cells.</p> </sec> <sec id="clr12054-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>To assess the early osteoinductive and cell–cell signaling events in adherent cells, commercially pure titanium implants (2.2 × 5 mm) with nanotopography (HF‐treated TiO<sub>2</sub> grit‐blasted) were compared with micron‐scale topography TiO<sub>2</sub> grit‐blasted (micron‐scale, control) implants <italic>in vivo</italic>. Six implants (<italic>n</italic> = 3/surface) were placed in 10 systemically healthy subjects and removed by reverse threading at 1, 3, and 7 days. Gene expression profiles of adherent cells were interrogated using low‐density RT‐PCR arrays.</p> </sec> <sec id="clr12054-sec-0003" sec-type="section"> <title>Results</title> <p>Osteoinduction was not observed at day 1 on either surface. At 3 days, elevated levels of BMP6, osteopontin, and osterix (OSX) were observed in RNA of cells adherent to both micron‐scale and nanotopography surfaces. Both surfaces supported osteoinductive gene expression at 7 days; however, modest elevations of most mRNAs and significantly higher OSX mRNA levels were measured for cells adhered to nanotopography implants. Further, chemokine and cytokine profiles including CXCL10, CXCL14, IL‐9, IL‐22, and TOLLIP were upregulated on nanotopographic surfaces as compared with microtopographic surfaces.</p> </sec> <sec id="clr12054-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Implants with superimposed nanoscale topography generate a greater induction of genes linked to osteogenesis and cell–cell signaling during the early phases of osseointegration.</p> </sec> </abstract>