Increases of ferrous iron oxidation activity and arsenic stressed cell growth by overexpression of Cyc2 in Acidithiobacillus ferrooxidans ATCC19859. (23rd August 2013)

• Record Type: Journal Article
• Authors: Liu, Wei; Lin, Jianqun; Pang, Xin; Mi, Shuang; Cui, Shuang; Lin, Jianqiang
• Is Part Of: Biotechnology and applied biochemistry. Volume 60:Number 6(2013:Nov./Dec.)
• Page Start: 623
• Page End: 628
• Publication Date: 2013-08-23
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1110
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p> <italic>Acidithiobacillus ferrooxidans</italic> plays an important role in bioleaching in reproducing the mineral oxidant of ferric iron (Fe³⁺) by oxidization of ferrous iron (Fe²⁺). The high‐molecular‐weight <italic>c</italic>‐type cytochrome Cyc2 that is located in the external membrane is postulated as the first electron carrier in the Fe²⁺ oxidation respiratory pathway of <italic>A. ferrooxidans</italic>. To increase ferrous iron oxidation activity, a recombinant plasmid pTCYC2 containing <italic>cyc2</italic> gene under the control of Ptac promoter was constructed and transferred into <italic>A. ferrooxidans</italic> ATCC19859. The transcriptional level of <italic>cyc2</italic> gene was increased by 2.63‐fold and Cyc2 protein expression was observed in the recombinant strain compared with the control. The ferrous iron oxidation activity and the arsenic stressed cell growth of the recombinant strain were also elevated.</p> </abstract>