High‐energy collision‐induced dissociation tandem mass spectrometry of regioisomeric lactose palmitic acid monoesters using matrix‐assisted laser desorption/ionization. (5th December 2013)

• Record Type: Journal Article
• Authors: Narouz, Mina R.; Soliman, Sameh E.; Fridgen, Travis D.; Nashed, Mina A.; Banoub, Joseph H.
• Is Part Of: Rapid communications in mass spectrometry. Volume 28:Number 2(2014)
• Page Start: 169
• Page End: 177
• Publication Date: 2013-12-05
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/rcm.6770
• Languages: English
• ISSNs: 0951-4198

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="rcm6770-sec-0001" sec-type="section"> <title>RATIONALE</title> <p>Structural characterization and differentiation of three newly synthesized lactose monopalmitate regioisomers at positions O‐3, O‐3' and O‐6' were realized by single‐stage matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight mass spectrometry (MALDI‐TOF/TOF‐MS) in the positive ion mode and by high‐energy collision‐induced dissociation tandem mass spectrometry (CID‐MS/MS).</p> </sec> <sec id="rcm6770-sec-0002" sec-type="section"> <title>METHODS</title> <p>A MALDI‐TOF/TOF analyzer was utilized for the analysis of these isobaric lactose monopalmitate regioisomers. The CID‐MS/MS spectra were acquired using high‐energy cid with a 2 kV potential difference between the source acceleration voltage and the collision cell.</p> </sec> <sec id="rcm6770-sec-0003" sec-type="section"> <title>RESULTS</title> <p>High‐energy (CID) tandem mass spectrometry (MS/MS<italic>)</italic> analyses of the sodiated molecules, [M + Na]<sup>+</sup>, showed distinguishing cross‐ring product ions and characteristic fingerprint product ions, which allowed the straight‐forward mass spectrometric characterization of these different regiosiomers.</p> </sec> <sec id="rcm6770-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>This investigation allowed us to unravel the novel fragmentation behavior of the sodiated regioisoimer molecules obtained from the mono‐substituted D‐lactose fatty acid esters using high‐energy CID‐TOF/TOF‐MS/MS analyses. The high‐energy CID of the [M + Na]<sup>+</sup> ions from the isobaric lactose monopalmitate regioiosmers promoted the formation of characteristic <sup>0, 2</sup>A<sub>2</sub> cross‐ring cleavage product ions. Copyright © 2013 John Wiley & Sons, Ltd.</p> </sec> </abstract>