Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells. Issue 5 (August 2014)

• Record Type: Journal Article
• Authors: Wang, Liying; Stueckle, Todd A.; Mishra, Anurag; Derk, Raymond; Meighan, Terence; Castranova, Vincent; Rojanasakul, Yon
• Is Part Of: Nanotoxicology. Volume 8:Issue 5(2014:Aug.)
• Page Start: 485
• Page End: 507
• Publication Date: 2014-08
• Subjects: Toxicology -- Periodicals; 615.9
• DOI: 10.3109/17435390.2013.801089
• Languages: English
• ISSNs: 1743-5390

Abstract

<abstract> <title>Abstract</title> <p>Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known about the long-term carcinogenic effect of CNT. Neoplastic transformation is a key early event leading to carcinogenesis. We studied the ability of single- and multi-walled CNTs to induce neoplastic transformation of human lung epithelial cells compared to asbestos. Long-term (6-month) exposure of the cells to occupationally relevant concentrations of CNT in culture caused a neoplastic-like transformation phenotype as demonstrated by increased cell proliferation, anchorage-independent growth, invasion and angiogenesis. Whole-genome expression signature and protein expression analyses showed that single- and multi-walled CNTs shared similar signaling signatures which were distinct from asbestos. These results provide novel toxicogenomic information and suggest distinct particle-associated mechanisms of neoplasia promotion induced by CNTs and asbestos.</p> </abstract>