Effect of multi-walled carbon nanotube surface modification on bioactivity in the C57BL/6 mouse model. Issue 3 (May 2014)

• Record Type: Journal Article
• Authors: Sager, Tina M.; Wolfarth, Michael W.; Andrew, Michael; Hubbs, Ann; Friend, Sherri; Chen, Teh-hsun; Porter, Dale W.; Wu, Nianqiang; Yang, Feng; Hamilton, Raymond F.; Holian, Andrij
• Is Part Of: Nanotoxicology. Volume 8:Issue 3(2014:May)
• Page Start: 317
• Page End: 327
• Publication Date: 2014-05
• Subjects: Toxicology -- Periodicals; 615.9
• DOI: 10.3109/17435390.2013.779757
• Languages: English
• ISSNs: 1743-5390

Abstract

<abstract> <title>Abstract</title> <p>The current study tests the hypothesis that multi-walled carbon nanotubes (MWCNT) with different surface chemistries exhibit different bioactivity profiles <italic>in vivo.</italic> In addition, the study examined the potential contribution of the NLRP3 inflammasome in MWCNT-induced lung pathology. Unmodified (BMWCNT) and MWCNT that were surface functionalised with -COOH (FMWCNT), were instilled into C57BL/6 mice. The mice were then examined for biomarkers of inflammation and injury, as well as examined histologically for development of pulmonary disease as a function of dose and time. Biomarkers for pulmonary inflammation included cytokines, mediators and the presence of inflammatory cells (IL-1β, IL-18, IL-33, cathepsin B and neutrophils) and markers of injury (albumin and lactate dehydrogenase). The results show that surface modification by the addition of the -COOH group to the MWCNT, significantly reduced the bioactivity and pathogenicity. The results of this study also suggest that <italic>in vivo</italic> pathogenicity of the BMWCNT and FMWCNT correlates with activation of the NLRP3 inflammasome in the lung.</p> </abstract>