Quantification of relative proportions of intact cells in microbiological samples using the example of Cryptosporidium parvum oocysts. (30th September 2013)

• Record Type: Journal Article
• Authors: Agulló‐Barceló, M.; Moss, J.A.; Green, J.; Gillespie, S.; Codony, F.; Lucena, F.; Nocker, A.
• Is Part Of: Letters in applied microbiology. Volume 58:Number 1(2014:Jan.)
• Page Start: 70
• Page End: 78
• Publication Date: 2013-09-30
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12157
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="graphical" xml:lang="en" id="lam12157-abs-0002"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>Significance and Impact of the Study:</bold> Whether in the context of microbial ecology or in an industrial context, many questions in microbiology are linked to microbial viability. As cultivation of micro‐organisms can be long or may not be possible, fast methods to assess the numbers of live cells are in great demand. We present here a straightforward strategy to determine the relative proportions of intact cells. The PCR‐based rapid method is expected to be useful where relative information is sufficient (e.g. for comparing the effect of different antimicrobial treatments on known numbers of micro‐organisms) or when the presence of PCR inhibitors does not allow absolute quantification. <boxed-text content-type="graphic" id="lam12157-blkfxd-0001" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg3zn327bz" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>