Paraoxonase 1 and oxidative stress in paediatric non‐alcoholic steatohepatitis. Issue 1 (13th September 2013)

• Record Type: Journal Article
• Authors: Desai, Sonal; Baker, Susan S.; Liu, Wensheng; Moya, Diana A.; Browne, Richard W.; Mastrandrea, Lucy; Baker, Robert D.; Zhu, Lixin
• Is Part Of: Liver international. Volume 34:Issue 1(2014)
• Page Start: 110
• Page End: 117
• Publication Date: 2013-09-13
• Subjects: Liver -- Periodicals; Liver -- Diseases -- Periodicals; 616.362
• DOI: 10.1111/liv.12308
• Languages: English
• ISSNs: 1478-3223

Abstract

<abstract abstract-type="main" xml:lang="en" id="liv12308-abs-0001"> <title>Abstract</title> <sec id="liv12308-sec-0001" sec-type="section"> <title>Background & Aims</title> <p>Non‐alcoholic steatohepatitis (NASH) in children is a significant public health concern. Oxidative stress is an important component in the pathophysiology of NASH. Several enzymatic antioxidant mechanisms protect the liver from oxidative injury. Examination of the expression of these enzymes in NASH livers may provide insight on the roles for these antioxidant mechanisms in the pathophysiology of NASH.</p> </sec> <sec id="liv12308-sec-0002" sec-type="section"> <title>Methods</title> <p>The mRNA expression of catalase, glutathione peroxidase 1 (GPX1), glutathione reductase (GSR), paraoxonase 1 (PON1) and other reactive oxygen species‐related genes was evaluated by microarray and quantitative real‐time PCR analyses. The PON1 protein levels were evaluated in liver and serum by Western blot analyses. Serum enzymatic activities of GPX, GSR and PON1 (paraoxonase and arylesterase activities) were examined.</p> </sec> <sec id="liv12308-sec-0003" sec-type="section"> <title>Results</title> <p>NASH livers exhibited elevated mRNA expression of catalase and PON1, but not GPX1 or GSR. No difference in serum GPX or GSR activity was detected between NASH patients and controls. Elevated expression of PON1 mRNA and protein was detected in NASH livers, but serum PON1 protein and activities were not elevated.</p> </sec> <sec id="liv12308-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Elevated expression of catalase and PON1 suggests protective roles for these antioxidants in NASH livers. Given the importance of oxidative stress in the pathophysiology of NASH, future studies focusing on these enzymes could identify important targets for therapeutic or preventive interventions for NASH patients.</p> </sec> </abstract>