User‐friendly markers linked to Fusarium wilt race 1 resistance Fw gene for marker‐assisted selection in pea. Issue 6 (4th September 2013)

• Record Type: Journal Article
• Authors: Kwon, Soon Jae; Smýkal, Petr; Hu, Jinguo; Wang, Meinan; Kim, Sung‐Jin; McGee, Rebecca J.; McPhee, Kevin; Coyne, Clarice J.; Link, W.
• Is Part Of: Plant breeding. Volume 132:Issue 6(2013)
• Page Start: 642
• Page End: 648
• Publication Date: 2013-09-04
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12085
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12085-abs-0001"> <title>Abstract</title> <p>Fusarium wilt is one of the most widespread diseases of pea. Resistance to Fusarium wilt race 1 was reported as a single gene, <italic>Fw</italic><italic>, </italic> located on linkage group III. The previously reported AFLP and RAPD markers linked to <italic>Fw</italic> have limited usage in marker‐assisted selection due to their map distance and linkage phase. Using 80 F₈ recombinant inbred lines (RILs) derived from the cross of Green Arrow × PI 179449, we amplified 72 polymorphic markers between resistant and susceptible lines with the target region amplified polymorphism (TRAP) technique. Marker–trait association analysis revealed a significant association. Five candidate markers were identified and three were converted into user‐friendly dominant SCAR markers. Forty‐eight pea cultivars with known resistant or susceptible phenotypes to Fusarium wilt race 1 verified the marker–trait association. These three markers, Fw_Trap_480, Fw_Trap_340 and Fw_Trap_220, are tightly linked to and only 1.2 cM away from the <italic>Fw</italic> locus and are therefore ideal for marker‐assisted selection. These newly identified markers are useful to assist in the isolation of the Fusarium wilt race 1 resistance gene in pea.</p> </abstract>