Rapid identification of Sporothrix schenckii in biopsy tissue by PCR. (20th December 2012)

• Record Type: Journal Article
• Authors: Liu, X.; Zhang, Z.; Hou, B.; Wang, D.; Sun, T.; Li, F.; Wang, H.; Han, S.
• Is Part Of: Journal of the European Academy of Dermatology and Venereology. Volume 27:Number 12(2013:Dec.)
• Page Start: 1491
• Page End: 1497
• Publication Date: 2012-12-20
• Subjects: Dermatology -- Periodicals; Sexually transmitted diseases -- Periodicals; 616.5
• DOI: 10.1111/jdv.12030
• Languages: English
• ISSNs: 0926-9959

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p> <bold>Background </bold> The dimorphic fungus <italic>Sporothrix schenckii</italic> is the etiological agent of sporotrichosis, an important cutaneous mycosis with a worldwide distribution. At present, it is challenging to rapidly discover and identify <italic>Sporothrix schenckii</italic> in biopsy tissues nowadays.</p> <p> <bold>Aims </bold> To explore new methods for rapid diagnosis of sporotrichosis.</p> <p> <bold>Materials and Methods </bold> We screened specific primers for <italic>Sporothrix schenckii</italic> using 50 clinical isolates from patients with sporotrichosis. DNA was extracted from the lesions of 30 cases of clinically suspected sporotrichosis using the Graham s method of CTAB and amplified by PCR using the screened specific primers.</p> <p> <bold>Results </bold> The primer S2‐R2 was applicable for the identification of <italic>S. schenckii</italic> from different geographic areas and clinical types with high specificity and sensitivity. Twenty‐five out of the thirty cases (83.3%) amplified using the primer S2‐R2 showed positive bands. Further positive bands were observed in 95.6% of cases tested positive by fungal culture.</p> <p> <bold>Conclusions </bold> Using the PCR technique and specific primers, we developed a new diagnostic method that can rapidly diagnose sporotrichosis with tissues obtained from clinical biopsies.</p> </abstract>