Effect of dietary carbohydrate on the growth performance, immune response, hepatic antioxidant abilities and heat shock protein 70 expression of Wuchang bream, Megalobrama amblycephala. (13th July 2013)

• Record Type: Journal Article
• Authors: Zhou, C.; Liu, B.; Ge, X.; Xie, J.; Xu, P.
• Is Part Of: Journal of applied ichthyology. Volume 29:Number 6(2013:Dec.)
• Page Start: 1348
• Page End: 1356
• Publication Date: 2013-07-13
• Subjects: Fishes -- Periodicals; Fish culture -- Periodicals; Fisheries -- Periodicals; Ichthyology -- Periodicals; 597
• DOI: 10.1111/jai.12264
• Languages: English
• ISSNs: 0175-8659

Abstract

<abstract abstract-type="main" id="jai12264-abs-0001"> <title>Summary</title> <p>To investigate the effects of different carbohydrate (CHO) levels in the diet of Wuchang bream <italic>Megalobrama amblycephala</italic>, the fish were randomly divided into six treatment groups. Five groups were fed 19, 25, 31, 38 and 47% CHO, respectively, for 8 weeks, and a control group was fed a diet with no CHO. Growth performance and feed utilization were significantly (P < 0.05) affected by the dietary carbohydrate level. Maximum weight gain and specific growth rate occurred at the 31% dietary carbohydrate level. Compared to the control, the 31% CHO group had a significantly increased serum total protein content, respiratory burst activity of leucocytes, serum complement 3 (C3) levels, serum lysozyme activity, serum alkaline phosphatase (AKP) activity and hepatic total antioxidative capacity (T‐AOC), as well as a decrease in serum glutamic‐oxaloacetic transaminase (GOT) activity. Compared to the control, the 47% CHO group had significantly increased serum GOT activity, and a tendency toward an increase in serum cortisol content and a decrease in serum lysozyme activity, hepatic superoxide dismutase (SOD) activity and T‐AOC. The relative level of hepatic heat shock protein 70 (HSP70) mRNA in fish fed the 38% CHO diet was significantly higher than those of fish fed the 19, 25 and 31% CHO diets, respectively (P<italic> </italic><<italic> </italic>0.05). After challenge with <italic>Aeromonas hydrophila</italic>, fish fed the 47% CHO had the significantly lowest post‐challenge survival, and fish fed the 31% CHO had the significantly highest post‐challenge survival (P<italic> </italic><<italic> </italic>0.05). The results of this study suggest that ingestion of excessive dietary CHO can impact the non‐specific immune responses, decrease the hepatic antioxidant abilities, and thus affect the health status of <italic>M. amblycephala</italic>.</p> </abstract>