An oral vaccine against candidiasis generated by a yeast molecular display system. Issue 3 (14th August 2013)

• Record Type: Journal Article
• Authors: Shibasaki, Seiji; Aoki, Wataru; Nomura, Takashi; Miyoshi, Ayuko; Tafuku, Senji; Sewaki, Tomomitsu; Ueda, Mitsuyoshi
• Is Part Of: Pathogens and disease. Volume 69:Issue 3(2013:Dec.)
• Page Start: 262
• Page End: 268
• Publication Date: 2013-08-14
• Subjects: Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1111/2049-632X.12068
• Languages: English
• ISSNs: 2049-632X

Abstract

<abstract abstract-type="main" id="fim12068-abs-0001"> <title>Abstract</title> <p>Enolase 1 (Eno1p) of <italic>Candida albicans</italic> is an immunodominant antigen. However, conventional technologies for preparing an injectable vaccine require purification of the antigenic protein and preparation of an adjuvant. To develop a novel type of oral vaccine against candidiasis, we generated <italic>Saccharomyces cerevisiae</italic> cells that display the Eno1p antigen on their surfaces. Oral delivery of the engineered <italic>S. cerevisiae</italic> cells prolonged survival rate of mice that were subsequently challenged with <italic>C. albicans</italic>. Given that a vaccine produced using molecular display technology avoids the need for protein purification, this oral vaccine offers a promising alternative to the use of conventional and injectable vaccines for preventing a range of infectious diseases.</p> </abstract>