Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl. Issue 6 (22nd December 2012)

• Record Type: Journal Article
• Authors: Lebarbenchon, Camille; Pantin‐Jackwood, Mary; Kistler, Whitney M.; Page Luttrell, M.; Spackman, Erica; Stallknecht, David E.; Brown, Justin D.
• Is Part Of: Influenza and other respiratory viruses. Volume 7:Issue 6(2013:Nov.)
• Page Start: 1237
• Page End: 1240
• Publication Date: 2012-12-22
• Subjects: Influenza -- Periodicals; Respiratory infections -- Periodicals; Virus diseases -- Periodicals; Influenza, Human -- Periodicals; Respiratory Tract Diseases -- Periodicals; Virus Diseases -- Periodicals; Grippe -- Périodiques; Appareil respiratoire -- Infections -- Périodiques; Maladies à virus -- Périodiques; 616.203
• DOI: 10.1111/irv.12070
• Languages: English
• ISSNs: 1750-2640

Abstract

<abstract abstract-type="main" id="irv12070-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (<italic>Anas platyrhynchos</italic>) and Pekin Ducks (<italic>Anas platyrhynchos domestica</italic>), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5‐infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.</p> </abstract>