ESX‐1‐induced apoptosis is involved in cell‐to‐cell spread of Mycobacterium tuberculosis. (2nd August 2013)

• Record Type: Journal Article
• Authors: Aguilo, J. I.; Alonso, H.; Uranga, S.; Marinova, D.; Arbués, A.; de, A.; Anel, A.; Monzon, M.; Badiola, J.; Pardo, J.; Brosch, Roland; Martin, Carlos
• Is Part Of: Cellular microbiology. Volume 15:Number 12(2013:Dec.)
• Page Start: 1994
• Page End: 2005
• Publication Date: 2013-08-02
• Subjects: Microbiology -- Periodicals; Cytology -- Periodicals; Host-parasite relationships -- Periodicals; Microbiology -- Periodicals; Cells -- Periodicals; Microbiologie -- Périodiques; Microbiologie; Relation hôte-parasite; Cytologie; Cellule; Réponse cellulaire; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); 579.05
• DOI: 10.1111/cmi.12169
• Languages: English
• ISSNs: 1462-5814

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Apoptosis modulation is a procedure amply utilized by intracellular pathogens to favour the outcome of the infection. Nevertheless, the role of apoptosis during infection with <italic>Mycobacterium tuberculosis</italic>, the causative agent of human tuberculosis, is subject of an intense debate and still remains unclear. In this work, we describe that apoptosis induction in host cells is clearly restricted to virulent <italic>M. tuberculosis</italic> strains, and is associated with the capacity of the mycobacteria to secrete the 6 kDa early secreted antigenic target ESAT‐6 bothunder <italic>in vitro</italic> and <italic>in vivo</italic> conditions. Remarkably, only apoptosis‐inducing strains are able to propagate infection into new cells, suggesting that apoptosis is used by <italic>M. tuberculosis</italic> as a colonization mechanism. Finally, we demonstrate that <italic>in vitro</italic> modulation of apoptosis affects mycobacterial cell‐to‐cell spread capacity, establishing an unambiguous relationship between apoptosis and propagation of <italic>M. tuberculosis.</italic> Our data further indicate that BCG and MTBVAC vaccines are inefficient in inducing apoptosis and colonizing new cells, correlating with the strong attenuation profile of these strains previously observed <italic>in vitro</italic> and <italic>in vivo</italic>.</p> </abstract>