AMP‐activated protein kinase as a promoting factor, but complement and thrombin as limiting factors for acquisition of cytoprotection: implications for induction of accommodation. (16th September 2013)

• Record Type: Journal Article
• Authors: Iwasaki, Kenta; Miwa, Yuko; Haneda, Masataka; Kuzuya, Takafumi; Ogawa, Haruko; Onishi, Akira; Kobayashi, Takaaki
• Is Part Of: Transplant international. Volume 26:Number 11(2013:Nov.)
• Page Start: 1138
• Page End: 1148
• Publication Date: 2013-09-16
• Subjects: Transplantation of organs, tissues, etc -- Periodicals; 617.95405
• DOI: 10.1111/tri.12186
• Languages: English
• ISSNs: 0934-0874

Abstract

<abstract abstract-type="main" id="tri12186-abs-0001"> <title>Summary</title> <p>Accommodation has been termed as a condition without graft rejection even in the presence of antidonor antibody. We previously reported an <italic>in vitro</italic> accommodation model, which demonstrated that preincubation of A/B antigen‐expressing endothelial cells with anti‐A/B antibody resulted in ERK inactivation followed by resistance to complement‐mediated cytotoxicity through the induction of complement regulatory genes. However, under the <italic>in vivo</italic> condition, the effects of complement and coagulation system cannot be ignored. The purpose of this study is to find effective ways to navigate accommodation by exploring the relevant signal transduction. Preincubation with a low level of complement or thrombin failed to induce resistance to complement‐mediated cytotoxicity. AMP‐activated protein kinase (AMPK) activators such as resveratrol, AICAR and metformin protected endothelial cells against complement‐mediated cytotoxicity through the increase in CD55, CD59, haem oxygenase‐1 (HO‐1) and ferritin heavy chain (ferritin H) genes, all of which were attenuated by AMPKα knock‐down. Resveratrol counteracted the inhibitory effect of pretreated complement and thrombin on acquisition of resistance to complement‐mediated cytotoxicity through AMPKα. AMPK regulation in endothelial cells could become the potential strategy to induce accommodation in clinical pro‐inflammation and pro‐coagulation.</p> </abstract>