Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: In vitro proliferative and multilineage potential assessment. (26th March 2013)

• Record Type: Journal Article
• Authors: Lange‐Consiglio, A.; Corradetti, B.; Meucci, A.; Perego, R.; Bizzaro, D.; Cremonesi, F.
• Is Part Of: Equine veterinary journal. Volume 45:Number 6(2013:Nov.)
• Page Start: 737
• Page End: 744
• Publication Date: 2013-03-26
• Subjects: Horses -- Diseases -- Periodicals; 636.108905
• DOI: 10.1111/evj.12052
• Languages: English
• ISSNs: 0425-1644

Abstract

<abstract abstract-type="main"> <title>Summary</title> <sec id="evj12052-sec-0001" sec-type="section"> <title>Reasons for performing study</title> <p>This is the first study comparing stemness features of equine mesenchymal progenitor cells derived from amniotic membrane and bone marrow.</p> </sec> <sec id="evj12052-sec-0002" sec-type="section"> <title>Objectives</title> <p>To investigate an alternative and noninvasive stromal cell source for equine tissue engineering.</p> </sec> <sec id="evj12052-sec-0003" sec-type="section"> <title>Study design</title> <p> <italic>In vitro</italic> experimental study of the characteristics of equine mesenchymal progenitor cells derived from amnion and bone marrow.</p> </sec> <sec id="evj12052-sec-0004" sec-type="section"> <title>Methods</title> <p>Cells isolated from amniotic membrane and bone marrow were analysed for proliferation (growth curve, doubling time, colony forming unit). Immunocytochemical detection of pluripotency markers and gene expression of stromal cell markers were also performed and these cells were studied for multilineage plasticity.</p> </sec> <sec id="evj12052-sec-0005" sec-type="section"> <title>Results</title> <p>Amniotic stromal cells (AMSCs) and bone marrow mesenchymal cells (BM‐MSCs) both exhibited mature stromal cell‐specific gene expression and immunocytochemical properties, but showed substantial differences in their proliferative and differentiation potential. The mean doubling time for AMSCs was significantly lower (P<0.05) than that observed for BM‐MSCs (1.17 ± 0.15 vs. 3.27 ± 0.19 days, respectively). Compared to AMSCs, BM‐MSCs also demonstrated a significantly (P<0.05) lower clonogenic capability (one fibroblast‐like colony forming unit from a mean of 590.15 cells seeded for BM‐MSCs vs. 242.73 cells seeded for AMSCs). BM‐MSCs did not differentiate into glial cells, and the osteogenic differentiation process was longer than for AMSCs.</p> </sec> <sec id="evj12052-sec-0006" sec-type="section"> <title>Conclusions and potential relevance</title> <p>The amniotic membrane could be a valuable source of MSCs to be used both for allogenic and/or autologous therapies. The noninvasive nature and low cost of collection, the rapid proliferation along with a greater differentiation potential and the 'off the shelf' preparation potential could make AMCs useful for cell therapy.</p> </sec> </abstract>