Influence of abiotic variables on culturable yeast diversity in two distinct Alpine glaciers. Issue 2 (2nd July 2013)

• Record Type: Journal Article
• Authors: Turchetti, Benedetta; Goretti, Marta; Branda, Eva; Diolaiuti, Guglielmina; D'Agata, Carlo; Smiraglia, Claudio; Onofri, Andrea; Buzzini, Pietro
• Is Part Of: FEMS microbiology ecology. Volume 86:Issue 2(2013)
• Page Start: 327
• Page End: 340
• Publication Date: 2013-07-02
• Subjects: Microbial ecology -- Periodicals; Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1574-6941.12164
• Languages: English
• ISSNs: 0168-6496

Abstract

<abstract abstract-type="main" id="fem12164-abs-0001"> <title>Abstract</title> <p>The influence of some abiotic variables (pH, dry weight, organic carbon, nitrogen and phosphorous) on culturable yeast diversity in two distinct, but adjacent Alpine glaciers (Glacier du Géant, France, and Miage Glacier, Italy) was investigated. In all, 682 yeast strains were isolated and identified by D1/D2 and ITS sequencing as belonging to species of the genera <italic>Aureobasidium</italic>, <italic> Candida</italic>, <italic> Bulleromyces</italic>, <italic> Cryptococcus</italic>, <italic> Cystofilobasidium</italic>, <italic> Dioszegia</italic>, <italic> Guehomyces</italic>, <italic> Holtermanniella</italic>, <italic> Leucosporidiella, Mrakia</italic>, <italic> Mrakiella</italic>, <italic> Rhodotorula</italic>, <italic> Sporidiobolus</italic>, <italic> Sporobolomyces</italic> and <italic>Udenyomyces</italic>. Overall, the most represented genera were <italic>Cryptococcus</italic> (55% of isolates), <italic>Rhodotorula</italic> (17%) and <italic>Mrakia</italic> (10%). About 10% of strains, presumably belonging to new species (yet to be described), were preliminarily identified at the genus level. Principal component analysis (PCA) revealed that organic carbon, nitrogen and phosphorous are apparently mostly related to culturable yeast abundance and diversity. In this context, the hypothesis that the frequency of isolation of certain species may be correlated with some organic nutrients (with special emphasis for phosphorous) is discussed.</p> </abstract>