Catalytic solid substrate–room temperature phosphorimetry for the determination of residual perphenazine based on the electronic effect of rhodamine 6G. (1st October 2012)

• Record Type: Journal Article
• Authors: Zheng, Zhi‐Yong; Cui, Ma‐Lin; Zhang, Li‐Hong; Jiang, Shu‐Lian; Jiao, Li; Lin, Xuan; Lin, Shao‐Qin; Liu, Jia‐Ming
• Is Part Of: Luminescence. Volume 28:Number 5(2013:Sep./Oct.)
• Page Start: 634
• Page End: 640
• Publication Date: 2012-10-01
• Subjects: Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.2408
• Languages: English
• ISSNs: 1522-7235

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>The rhodamine 6G<sup>+</sup>‐perphenazine (Rhod 6G<sup>+</sup>–PPH) compound is formed in the ester‐exchange reaction between ‐OH of PPH and ‐COOC<sub>2</sub>H<sub>5</sub> of Rhod 6G<sup>+</sup>. PPH was oxidized to a red compound (PPH') in the presence of K<sub>2</sub>S<sub>2</sub>O<sub>8</sub>. Interestingly, the room temperature phosphorescence (RTP) of Rhod 6G<sup>+</sup> was quenched because the ‐OH of PPH′ reacted with ‐COOC<sub>2</sub>H<sub>5</sub> of Rhod 6G<sup>+</sup>–PPH to form Rhod 6G<sup>+</sup>–PPH' and PPH, which decreased the π‐electron density (δ) of the carbon atom in the Rhod 6G<sup>+</sup>–PPH' conjugated system and enhanced the nonradiation energy loss of the excited Rhod 6G<sup>+</sup> of the triplet state. The PPH content was directly proportional to the Δ<italic>I</italic><sub>p</sub> of the system. Thus, a new catalytic solid‐substrate room temperature phosphorimetry (SSRTP) method was established for the determination of PPH. The method had high sensitivity (the limit of detection was 0.019 fg/spot, corresponding to a concentration of 4.8 × 10<sup>–14</sup> g/mL; the sampling quantity was 0.40 μL/spot), good selectivity, convenience and speed. The analytical results were in accordance with those of high‐performance liquid chromatography (HPLC). The structures of Rhod 6G<sup>+</sup>, PPH and Rhod 6G<sup>+</sup>–PPH were characterized by infrared spectra. The reaction mechanism by which PPH was determined is discussed. Copyright © 2012 John Wiley & Sons, Ltd.</p> </abstract>