Injectable microsphere/hydrogel hybrid system containing heat shock protein as therapy in a murine myocardial infarction model. (November 2013)

• Record Type: Journal Article
• Authors: Lee, Jangwook; Cha, Min-Ji; Lim, Kwang Suk; Kim, Jang-Kyung; Lee, Sang-Kyung; Kim, Yong-Hee; Hwang, Ki-Chul; Lee, Kuen Yong
• Is Part Of: Journal of drug targeting. Volume 21:Number 9(2013)
• Page Start: 822
• Page End: 829
• Publication Date: 2013-11
• Subjects: Drug delivery systems -- Periodicals; Drug Delivery Systems; Vehicles; Drug Administration Routes; Drug Evaluation; 615.7
• DOI: 10.3109/1061186X.2013.829072
• Languages: English
• ISSNs: 1061-186X

Abstract

<abstract> <title>Abstract</title> <p>Heat shock proteins, acting as molecular chaperones, protect heart muscle from ischemic injury and offer a potential approach to therapy. Here we describe preparation of an injectable form of heat shock protein 27, fused with a protein transduction domain (TAT-HSP27) and contained in a hybrid system of poly(<sc>d</sc>, <sc>l</sc>-lactic-<italic>co</italic>-glycolic acid) microsphere and alginate hydrogel. By varying the porous structure of the microspheres, the release of TAT-HSP27 from the hybrid system was sustained for two weeks <italic>in vitro</italic>. The hybrid system containing TAT-HSP27 was intramyocardially injected into a murine myocardial infarction model, and its therapeutic effect was evaluated <italic>in vivo</italic>. The sustained delivery of TAT-HSP27 substantially suppressed apoptosis in the infarcted site, and improved the ejection fraction, end-systolic volume and maximum pressure development in the heart. Local and sustained delivery of anti-apoptotic proteins such as HSP27 using a hybrid system may present a promising approach to the treatment of ischemic diseases.</p> </abstract>