Expression, purification, crystallization and preliminary crystallographic analysis of spermidine acetyltransferase from Escherichia coli. Issue 8 (31st July 2013)

• Record Type: Journal Article
• Authors: Niiyama, Mayumi; Sugiyama, Shigeru; Hirose, Mika; Ishikawa, Sae; Tomitori, Hideyuki; Higashi, Kyohei; Yamashita, Tomoko; Adachi, Hiroaki; Takano, Kazufumi; Murakami, Satoshi; Murata, Michio; Inoue, Tsuyoshi; Mori, Yusuke; Kashiwagi, Keiko; Matsumura, Hiroyoshi; Igarashi, Kazuei
• Is Part Of: Acta crystallographica. Volume 69:Issue 8(2013:Aug.)
• Page Start: 884
• Page End: 887
• Publication Date: 2013-07-31
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S1744309113017132
• Languages: English
• ISSNs: 1744-3091

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The spermidine acetyltransferase (SAT) from <italic>Escherichia coli</italic> catalyses the transfer of acetyl groups from acetyl‐CoA to spermidine. SAT has been expressed and purified from <italic>E. coli</italic>. SAT was crystallized by the sitting‐drop vapour‐diffusion method to obtain a more detailed insight into the molecular mechanism. Preliminary X‐ray diffraction studies revealed that the crystals diffracted to 2.5 Å resolution and belonged to the cubic space group <italic>P</italic>23, with unit‐cell parameters <italic>a</italic> = <italic>b</italic> = <italic>c</italic> = 148.7 Å. They contained four molecules per asymmetric unit.</p> </abstract>